- Regulatory Status
- RUO
- Other Names
- Small inducible cytokine subfamily B, member 8 (SCYB8), Monocyte derived neutrophils chemotactic factor (MDNCF), Neutrophil-activating peptide 1 (NAP1), Granulocyte chemotactic protein 1 (GCP1), Chemochine, CXC motif, ligand 8, CXCL8
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Bioactivity was measured by its property to chemoattract human peripheral blood neutrophils in a dose dependent manner with an ED50 = 1 to 5 ng/ml.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 574202 | 10 µg | $147.00 | |||
| 574204 | 25 µg | $229.00 | |||
| 574206 | 100 µg | $680.00 | |||
IL-8 (CXCL8) was originally recognized as a neutrophil-activating protein on the basis of two in-vitro effects, chemotaxis and the release of granule enzymes. IL-8 is generated as a precursor of 99 amino acids. Once the mature cytokine is secreted, the amino-terminal is processed to yield several biologically active variants. The predominant variant consists of 72 amino acids. IL-8 is one of the ELR (Glu-Leu-Arg) motif-positive CXC-chemokines and shares similarities with angiogenic cytokines.
Product Details
- Source
- Human IL-8, amino acids Ser28-Ser99 (Accession # NM_000584) was expressed in E. coli.
- Molecular Mass
- The 72 amino acid recombinant protein has a predicted molecular mass of approximately 8385 Da. The DTT-reduced protein migrates at approximately 8 kD and the non-reduced protein migrates at approximately 10 kD by SDS-PAGE. The N-terminal amino acid is Serine.
- Purity
- >95%, as determined by Coomassie stained SDS-PAGE
- Formulation
- 0.22 µm filtered protein solution is in PBS
- Endotoxin Level
- Less than 0.01 ng per µg cytokine as determined by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Bioactivity was measured by its property to chemoattract human peripheral blood neutrophils in a dose dependent manner with an ED50 = 1 to 5 ng/ml.
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
- Product Citations
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Antigen Details
- Structure
- Chemokine
- Distribution
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IL-8 is expressed by neutrophils, mococytes, macrophages, mast cells, endothelial cells, fibroblasts, keratinocytes, synovial cells, chondrocytes, epithelial cells, megakariocytes, melanocytes, hepatocytes, mesangial, and tumor cells. Most primary and metastatic solid tumors, such as breast, uterine, prostate, colon, and pancreatic carcinomas, melanoma, and glioblastoma, constitutively express IL-8.
- Function
- CXCL8 has diverse functions in immune surveillance, inflammation, and angiogenesis. CXCL8 directly modulates endothelial cell proliferation and migration, thus promoting angiogenesis. IL-8 modulates myelopoiesis by suppressing the myeloid progenitor proliferation. IL-8 also modulates the homing of hematopoitic progenitors by affecting the secretion of MMPs and TIMPs from bone marrow and peripheral blood CD34+ cells. Monocytes and macrophages generate IL-8 upon stimulation with IL-1α, IL-1β, TNFα, IL-3, GMCSF, endotoxin, lectins, phorbol esters, immune complexes, and phagocytosis. Opsonized particles are also a prime stimulus for IL-8 production by neutrophils.
- Interaction
- Neutrophils, basofils, highly cytotoxic CD8 T cells, megakariocytes, endothelial cells.
- Ligand/Receptor
- CXCR1 (CDw128a) and CXCR2
- Biology Area
- Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- Cytokines/Chemokines
- Antigen References
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1. Hess C, et al. 2004. Blood 104:3463.
2. Emadi S, et al. 2005. Blood 105:464.
3. Matsuo Y, et al. 2009. Int. J. Cancer 4:853.
4. Drewniak A, et al. 2010. Blood 115:4588. - Gene ID
- 3576 View all products for this Gene ID
- UniProt
- View information about IL-8 on UniProt.org