- Regulatory Status
- RUO
- Other Names
- P40, MEA (mast cell growth enhancing activity), MCGF (Mast cell growth factor), TCGF-3 (T cell growth factor-3)
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 715502 | 10 µg | $253.00 | |||
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IL-9 was initially identified in mice as a T cell growth factor. Human and murine IL-9 show a 56% amino acid sequence homology. IL-9 is a cytokine that acts as a regulator of a variety of hematopoietic cells, stimulates cell proliferation, and prevents apoptosis. The IL-9 receptor consists of the cytokine-specific IL-9 receptor α-chain (IL-9Rα) and the common γ-chain that is shared by IL-2, IL-4, IL-7, IL-15, and IL-21. IL-9 is expressed by activated Th9, Th17, Tregs, and mast cells and appears to play an important role in the development and function of subsets within the CD4+ T cell lineage. IL-9 also inhibits immune responses by enhancing the suppressive properties of Tregs and by recruiting immunosuppressive mast cells to sites of inflammation. Genetic studies on a mouse model of asthma demonstrated that this cytokine is a key factor in the pathogenesis of bronchial hyperresponsiveness. In this sense, it has been described that IL-9 and IL-9R expression are increased in lungs of asthmatic patients. In addition, IL-9 promotes mast cell mediated intestinal permeability and plays a role in the development of food allergies. IL-9 has also been associated with atopic dermatitis.
Product Details
- Source
- Human IL-9, amino acids (Gln19-Ile144) (Accession# NP_000581.1), was expressed in E. coli.
- Molecular Mass
- The 127 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 14.2 kD. The predicted N-terminal amino acid is Met.
- Purity
- >98%, as determined by Coomassie stained SDS-PAGE and HPLC analysis.
- Formulation
- Lyophilized
- Endotoxin Level
- Less than 0.1 ng per µg of protein.
- Storage & Handling
- Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. It is recommended to further dilute in a buffer, such as 5% Trehalose, and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
- Activity
- ED50 is ≤ 0.2 ng/ml, corresponding to a specific activity of ≥ 5.0 x 106 units/mg as determined by the dose-dependent stimulation of the proliferation of human M07e cells.
- Application
-
Bioassay
Antigen Details
- Structure
- Cytokine
- Distribution
- Th2 cells and mast cells, activated Th9, Th17, and Tregs.
- Function
- Autocrine growth and activation factor for Th17, Tregs, and mast cells. IL-1, histamine, and LPS induce the expression of IL-9 in mast cells. IL-9 is induced synergistically by IL-2, IL-4, and TGF-β and inhibited by IFN-γ in naïve CD4 T cells.
- Interaction
- T helper cells, Th9, Th17, Treg, human airway smooth muscle cells.
- Ligand/Receptor
- IL-9R complex (IL-9R α and γ chain).
- Bioactivity
- Human IL-9 induces proliferation of M07e cells.
- Cell Type
- Hematopoietic stem and progenitors
- Biology Area
- Cell Biology, Stem Cells, Immunology
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Goswami R, and Kaplan MH. 2011. J. Immunol. 186:3283.
2. Kaiko GE, and Foster PS. 2011. Curr. Opin. Allergy Clin. Immunol. 11:39.
3. Townsend JM, et al. 2000. Immunity 13:573.
4. Mock BA, et al. 1990. Immunogenetics. 31:265.
5. Tan C. et al. 2010. J. Immunol. 185:6795.
6. Jager A. et al. 2009. J. Immunol. 183:7169.
7. Sismanopoulos N, et al. 2012. PLoS One 7:e33271. - Gene ID
- 3578 View all products for this Gene ID
- UniProt
- View information about IL-9 on UniProt.org