- Regulatory Status
- RUO
- Other Names
- P40, MEA (mast cell growth enhancing activity), MCGF (Mast cellgrowth factor), TCGF-3 (T cell growth factor-3)
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Human IL-9 induces proliferation of M07e cells in a dose-dependent manner. The ED50 for this effect is 0.008– 0.12 ng/mL. -
Stability Testing for Recombinant Human IL-9. Recombinant human IL-9 was aliquoted in PBS and pH 7.2 at 0.2 mg/mL. One aliquot was kept at 4°C (Control), and another was frozen and thawed four times (4x Freeze/Thaw). The samples were tested by their ability to induce the proliferation of M07e cells in a dose-dependent manner. The ED50 for this effect is 0.008– 0.12 ng/mL.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 578804 | 25 µg | $315.00 | |||
| 578806 | 100 µg | $1095.00 | |||
Interleukin-9 (IL-9) was initially identified in mice as a T cell growth factor. Human and murine IL-9 show a 56% amino acid sequence homology. IL-9 is a cytokine that acts as a regulator of a variety of hematopoietic cells, stimulates cell proliferation, and prevents apoptosis. The IL-9 receptor consists of the cytokine-specific IL-9 receptor α-chain (IL-9Rα) and the common γ-chain that is shared by IL-2, IL-4, IL-7, IL-15 and IL-21. IL-9 is expressed by activated Th9, Th17, Treg, and mast cells and appears to play important roles in the development and function of subsets within the CD4+ T cell lineage. IL-9 also inhibits immune responses by enhancing the suppressive properties of Tregs and by recruiting immune suppressive mast cells to sites of inflammation. Genetic studies on a mouse model of asthma demonstrated that IL-9 plays a key role in the pathogenesis of bronchial hyperresponsiveness correlating with the observed increased expression of IL-9 and IL-9R in lungs of asthmatic patients. In addition, IL-9 promotes mast cell mediated intestinal permeability, development of food allergies and atopic dermatitis.
Product Details
- Source
- Recombinant human IL-9, amino acid Gln19-Ala144 (Accession No. P15248) was expressed in E.coli.
- Molecular Mass
- The 126 amino acid recombinant protein has a predicted molecular mass of approximately 14.1 kD. The DTT-reduced and non-reduced protein migrate at approximately 7 - 15 kD by SDS-PAGE. The N-terminal amino acid is Gln.
- Purity
- > 95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS, pH 7.2
- Concentration
- 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- ED50 = 0.008 – 0.12 ng/mL, as determined by a dose-dependent stimulation in a proliferation assay with M07e cells. Deep Blue Cell Viability™ Kit (Cat. No. 424701) is used to measure the proliferation.
- Application
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Bioassay
- Application Notes
-
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Monomer
- Distribution
-
Th2 cells, mast cells, activated Th9, Th17, and Treg cells
- Function
- Autocrine growth and activation factor for Th17, Tregs, and mast cells. IL-1, histamine, and LPS induce the expression of IL-9 in mast cells. IL-9 is induced synergistically by IL-2, IL-4, and TGFβ and inhibited by IFNγ in naïve CD4 T cells
- Interaction
- T helper cells, Th9, Th17, Tregs, human airway smooth muscle cells
- Ligand/Receptor
- IL-9R complex (IL-9Rα and γ chain)
- Bioactivity
- Mouse IL-9 induces proliferation of M07e cells
- Cell Type
- Hematopoietic stem and progenitors
- Biology Area
- Cell Biology, Immunology, Stem Cells
- Molecular Family
- Cytokine/Chemokine Receptors
- Antigen References
-
- Goswami R and Kaplan MH. 2011. J Immunol. 186:3283-8.
- Kaiko GE and Foster PS. 2011. Curr Opin Allergy Clin Immunol. 11:39-45.
- Townsend JM. et al. 2000. Immunity. 13:573-83.
- Mock BA. et al. 1990. Immunogenetics. 31:265-70.
- Tan C. et al. 2010. J Immunol. 185:6795-801.
- Jager A. et al. 2009. J Immunol. 183:7169-77.
- Sismanopoulos N, et al. 2012. PLoS One. 7: e33271.
- Ye D. et al. 2020. Life Sci. 255:117844.
- Gene ID
- 3578 View all products for this Gene ID
- UniProt
- View information about IL-9 on UniProt.org