- Regulatory Status
- RUO
- Other Names
- INPP5F, MSTPO47, SAC2, hSAC2, MSTP007, inositolpolyphosphate-5-phosphatase F, Sac Domain- Containing Phosphoinositide 4-Phosphatase 2, Sac Domain-Containing Inositol Phosphatase 2, Phosphatidylinositide Phosphatase SAC2, KIAA0966
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Stability Testing for Recombinant Human INPP5F. One aliquot (Control) was stored at 4°C for 7 days, while another aliquot (4XF/T) was subjected to multiple freeze-thaw cycles by freezing at -80°C and thawing four times over a 7-day period. Each freeze-thaw cycle involved placing the sample on ice and slowly thawing it until no ice crystals remained in the tube. A minimum of 24 hours elapsed between each freeze and thaw event. Following treatment, the reduced samples (4 µg/lane) were separated by SDS-PAGE and stained with Coomassie brilliant blue dye. Lane M: Protein ladder (Cat. No. 773302).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 569106 | 100 µg | $300.00 | |||
Inositol polyphosphate 5-phosphatase F (INPP5F), also known as phosphatidylinos phosphatidylinositide phosphatase itide phosphatase SAC2, is a member of INPP5 family. A key role of INPP5F is regulation of polyphosphoinositide metabolism and phosphoinositide signaling pathways by regulating the levels of inositol phospholipids. Positioned in the cytoplasm, INPP5F is closely associated with cellular membranes and participates in various cellular processes, including membrane trafficking, cytoskeleton dynamics, and signal transduction.
Mechanistically, INPP5F activates the Notch signaling pathway and upregulates c-MYC and cyclin E1 in hepatocellular carcinoma by interacting with ASPH. INPP5F also modulates the Akt/GSK-3β pathway by reducing Akt and GSK-3β phosphorylation levels. INPP5F is expressed in various tissues including heart, kidney, skeletal muscle, and brain. INPP5F contributes to tumorigenesis and tumor progression. INPP5F rs117896735 variant has been associated with an increased risk of Parkinson’s Disease.
Product Details
- Source
- Human INPP5F, amino acids (Arg593-Ile760) (Accession #Q9Y2H2-1) with a N-terminal 6His tag was expressed in insect cells
- Molecular Mass
- The 197 amino acid recombinant protein has a predicted molecular mass of approximately 23.11 kD. The DTT-reduced and non-reduced protein migrates at approximately 20-30 kD by SDS-PAGE.
- Purity
- > 85% as determined by Coomassie stained SDS-PAGE.
- Formulation
- Protein is formulated in 50 mM HEPES, 300 mM NaCl, 5% glycerol, 1mM TCEP, pH 7.5
- Concentration
- 100 μg at 0.5 mg/mL
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 1 week, at -20°C for up to three months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Application
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Standard
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped with dry ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Monomer
- Distribution
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INPP5F is highly expressed in the heart, brain, and skin tissues
- Function
- INPP5F regulates endocytic recycling, attenuates heart hypertrophy, and participates in the onset of Parkinson’s disease 23
- Interaction
- Notch1, CRM1, ASPH
- Cell Type
- Endothelial cells, Epithelial cells, Neurons
- Biology Area
- Cell Proliferation and Viability, Immuno-Oncology, Neurodegeneration, Neuroinflammation, Signal Transduction
- Molecular Family
- Protein Kinases/Phosphatase
- Antigen References
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- Hong SK, et al. 2014. Sci. Rep. 4:7330.
- Palermo G, et al. 2015. Blood Cancer J. 5:e353.
- Zhu W, et al. 2009. Circ Res. 105:1240-7.
- Xiaolei Z, et al. 2020. J Cell Mol Med. 24:9898-9907.
- Qianlei Z, et al. 2022. J Exp Clin Cancer Res. 41:13.
- Ooms LM, et al. 2015. Cancer Cell. 28:155-69.
- Ramos AR, et al. 2019. J Lipid Res. 60:276-286.
- Gene ID
- 22876 View all products for this Gene ID
- UniProt
- View information about INPP5F on UniProt.org