- Regulatory Status
- RUO
- Other Names
- Keratinocyte growth factor, Fibroblast growth factor 7 (FGF-7), HBGF-7
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 711702 | 10 µg | $253.00 | |||
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KGF is a heparin binding growth factor secreted by cells of mesenchymal origin. KGF acts in a paracrine manner by binding exclusively to a splicing variant of FGF receptor 2, FGFR2-IIIB. FGFR-IIIB is predominatly expressed by epithelial cells. This suggests that KGF may play an important role in mesenchymal-epithelial communication. KGF can stimulate proliferation, migration, and differentiation of epithelial cells in a variety of tissues. In skin, KGF can be strongly upregulated in dermal fibroblasts after skin injury. KGF can induce keratinocyte proliferation and migration. KGF can also protect epithelial cells from reactive oxygen species. In liver, KGF is required for adult liver regeneration. In brain, KGF is important for inhibitory synapse formation in the hippocampus. KGF-deficient mice are prone to epileptic seizures induced by chemical kindling in adults. KGF signaling is also important for hair follicle development. Injection of recombinant KGF into adult athymic nude mice can induce dose-dependent hair growth. In lung, KGF can protect animals from oxidant-induced injury. Taken together, KGF is a potential target for epithelial tissue repair.
Product Details
- Source
- Human KGF, amino acids Cys32-Thr194 (Accession# Q6FGV5), was expressed in E. coli.
- Molecular Mass
- The 163 amino acid recombinant protein has a predicted molecular mass of approximately 18.9 kD. The predicted N-terminal amino acid is Cys.
- Purity
- >95%, as determined by Coomassie stained SDS-PAGE and HPLC analysis.
- Formulation
- Lyophilized, carrier-free.
- Endotoxin Level
- Less than 0.1 ng per µg of protein.
- Storage & Handling
- Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in 1x PBS to a concentration of 0.5-1.0 mg/ml. Do not vortex. It is recommended to further dilute in a buffer, such as 5% Trehalose, and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
- Activity
- The ED50 as determined by the dose-dependent stimulation of thymidine uptake by KGF-responsive BaF3 cells is ≤ 10 ng/ml corresponding to a specific activity of ≥ 1 x 105 units/mg.
- Application
-
Bioassay
- Product Citations
-
Antigen Details
- Structure
- Growth factor
- Distribution
- KGF is widely expressed among cells of mesenchymal (stromal) origin.
- Function
- KGF is secreted by fibroblast and can stimulate proliferation of epithelial cells. KGF expression can be highly upregulated in various tissues after injury.
- Interaction
- Epithelial cells
- Ligand/Receptor
- FGF-7 receptor (FGFR2/IIIb).
- Cell Type
- Embryonic Stem Cells
- Biology Area
- Cell Proliferation and Viability, Stem Cells
- Molecular Family
- Growth Factors, Cytokines/Chemokines
- Antigen References
-
1. Werner S, et al. 1998. Cytokine Growth Factor Rev. 9:153.
2. Werner S, et al. 1994. Science. 266:819.
23. Richardson GD, et al. 2009. Development. 136:2153.
24. Yen TT, et al. 2014. Protein Pept. Lett. 21:306.
25. Hsu YR, et al. 1999. Biochemistry. 38:2523. - Gene ID
- 2252 View all products for this Gene ID
- UniProt
- View information about KGF on UniProt.org