- Regulatory Status
- RUO
- Other Names
- SELL, CD62 antigen-like family member L, CD62L, Leukocyte-endothelial cell adhesion molecule 1 (LECAM1), Leukocyte adhesion molecule 1 (LAM-1), Leukocyte surface antigen Leu-8, Lymph node homing receptor, TQ1, gp90-MEL, Lymphocyte antigen 22 (Ly-22)
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Immobilized recombinant human L-Selectin induces adhesion of U937 cells in a dose dependent manner. ED50 for this effect is 0.5 - 2.0 µg/mL. Calcein-AM (Cat. No. 425201) was used to measure the number of adherent cells. -
Stability testing for human L-Selectin. Human L-Selectin was aliquoted in PBS, pH 7.2 at 0.2 mg/ml. One aliquot was freeze and thawed four times (4x freeze/thaws), and compared to a control kept at 4°C (control). The samples were tested in U937 cell adhesion assay.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 772702 | 10 µg | $83.00 | |||
| 772704 | 25 µg | $147.00 | |||
| 772706 | 100 µg | $364.00 | |||
| 772708 | 500 µg | $1172.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
L-selectin, belonging to the selectin/LECAM family, is a type I transmembrane protein, consisted of one C-type lectin domain and EGF-like repeats, followed by a transmembrane region and a short cytoplasmic tail of 17 amino acids. L-selectin gene encodes a protein with a predicted size of 42 kD, but the actual molecular weight is around 70 kD to 120 kD, due to N-linked glycosylation. L-selectin is constitutively expressed on leukocytes. As a homing receptor, L-selectin mediates adhesion and rolling of leukocytes on endothelial cells, in turn leukocytes enter peripheral lymphoid organs via high endothelial venules (HEV). L-selectin also directs leukocytes to inflammation sites. Circulating leukocytes can also tether on adherent leukocytes through the interaction between L-selectin and P-selectin glycoprotein ligand-1 (PSGL-1). L-selectin also interacts with mucins, such as CD34, glycosylated cell adhesion molecule-1 (GlyCAM-1) and podocalyxin. Ligand-induced clustering of L-selectin enhances leukocyte tethering and causes signaling cascade, promoting activation of integrin and mobilizing chemokine receptor on cell surface. To negatively regulate leukocyte recruitment and migration, L-selectin is cleaved by ADAM17, released from cell surface (ectodomain shedding) with functional activity. High level of soluble L-selectin can be detected in human plasma.
Product Details
- Source
- Human L-Selectin, amino acids (Trp39 - Asn332) (Accession#: P14151), with a C-terminal human IgG1 Fc and 6xHis tag, was expressed in 293E cells.
- Molecular Mass
- The 535 amino acid recombinant protein has a predicted molecular mass of approximately 60.19 kD. The DTT-reduced and non-reduced protein migrates at approximately 75 and 150 kD respectively by SDS-PAGE. The predicted N-terminal amino acid is Trp.
- Purity
- >95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS, pH 7.2.
- Endotoxin Level
- Less than 0.1 EU per µg protein as determined by the LAL method.
- Concentration
- 10-25 µg sizes are bottled at 200 µg/mL. 100 µg and larger sizes and larger are bottled at the concentration indicated on the vial.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- ED50 = 0.5 - 2 µg/mL as measured by the ability of immobilized protein to support the adhesion of U937 cells. Calcein-AM (Cat. No. 425201) was used to measure the number of adherent cells.
- Application
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Bioassay
- Application Notes
-
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Adhesion molecule
- Distribution
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Lymphoid tissues, leukocytes, cell surface and plasma.
- Function
- L-Selectin mediates leukocyte rolling and homing via HEV.
- Interaction
- Endothelial cells of high endothelial venules (HEVs), activated endothelial cells, and leukocytes.
- Ligand/Receptor
- P-selectin glycoprotein ligand-1 (PSGL-1), PODXL2, and mucins, such as CD34, GlyCAM-1, podocalyxin.
- Bioactivity
- Supports adhesion of U937 cells.
- Biology Area
- Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
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- McEver RP. 2002. Curr. Opin. Cell Biol. 14:581-6.
- Rosen SD. 2004. Annu. Rev. Immunol. 22:129-56.
- Giblin PA, et al. 1997. J. Immunol. 159:3498-507.
- Ding Z, et al. 2003. Blood 101:4245-52.
- Dwir O, et al. 2002. J. Biol. Chem. 277:21130-9.
- Martinez M, et al. 2004. J. Biol. Chem. 280:5378-90.
- Ridger VC, et al. 2005. Am. J. Pathol. 166:945-52.
- Lasky LA, et al. 1992. Cell. 69:927-38.
- Puri KD, et al. 1995. J. Cell. Biol. 131:261-70.
- Kerr SC, et al. 2008. J. Immunol. 181:1480-90.
- Rosen SD. 2004. Annu. Rev. Immunol. 22:129-56.
- Schleiffenbaum B, et al. 1992. J. Cell. Biol. 119:229-38.
- Li Y, et al. 2006. Blood. 108:2275-9.
- Gene ID
- 6402 View all products for this Gene ID
- UniProt
- View information about L-Selectin on UniProt.org