- Regulatory Status
- RUO
- Other Names
- CSF1, CSF-1, MCSF
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 716602 | 10 µg | $253.00 | |||
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M-CSF was first characterized as a glycoprotein that induces monocyte and macrophage colony formation from precursors in murine bone marrow cultures. M-CSF is constitutively present at biologically active concentrations in human serum. It binds CD14+ monocytes and promotes the survival/proliferation of human peripheral blood monocytes. In addition, M-CSF enhances inducible monocyte functions including phagocytic activity, microbial killing, cytotoxicity for tumor cells as well as synthesis of inflammatory cytokines such as IL-1, TNF-α, and IFN-γ in monocytes. M-CSF induces RANKL production in mature human osteoclasts; consequently, M-CSF is a potent stimulator of mature osteoclast resorbing activity. Also, M-CSF induces VEGF in monocytes in human tumors. High levels of M-CSF, mononuclear phagocytes, and VEGF are associated with poor prognosis in patients with cancer. High levels of M-CSF have been associated with different pathologies such as pulmonary fibrosis and atherosclerosis. M-CSF binds to its receptor M-CSFR, and this receptor is shared by a second ligand, IL-34. Human M-CSF and IL-34 exhibit cross-species specificity, both bind to human and mouse M-CSF receptors.
Product Details
- Source
- Human M-CSF, amino acids Glu33-Ser190 (Accession# NM_172212.2) with N terminus Met was expressed in E. coli.
- Molecular Mass
- The 159 amino acid N-terminal methionylated recombinant protein has a predicted molecular weight of 18.5 kD. The predicted N-terminal amino acid is Met. Recombinant human M-CSF is a 37 kD homodimeric protein.
- Purity
- >98%, as determined by Coomassie stained SDS-PAGE and HPLC analysis.
- Formulation
- Lyophilized, carrier-free.
- Endotoxin Level
- Less than 0.1 ng per µg of protein.
- Storage & Handling
- Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. It is recommended to further dilute in a buffer, such as 5% Trehalose, and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
- Activity
- ED50 is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1.0 x 106 units/mg as determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells.
- Application
-
Bioassay
- Application Notes
-
This product is reactive with human, monkey, mouse, and rat.
Antigen Details
- Structure
- Growth factor, Disulfide-linked glycosylated homodimer
- Distribution
- M-CSF is broadly expressed in adult mouse tissues. M-CSF is released by fibroblasts, breast cancer cell lines, alveolar macrophages, stromal bone marrow cells, endothelial cells, and mesenchymal cells.
- Function
- M-CSF is the key regulator of the survival, proliferation, and differentiation of mononuclear phagocytes and plays a central role in the regulation of osteoclastogenesis. CSF-1 also regulates the development of Paneth cells, Langerhans cells, lamina propria dendritic cells, and microglia.
- Interaction
- Monocytes, macrophages, mononuclear phagocyte precursors, microglia, proliferating smooth muscle cells, umbilical vein endothelial cells, and breast cancer cell lines.
- Ligand/Receptor
- M-CSFR or CSF1R (CD115)
- Bioactivity
- Human M-CSF is able to induce proliferation of mouse M-NSF-60 cells.
- Cell Type
- Hematopoietic stem and progenitors, Embryonic Stem Cells
- Biology Area
- Cell Biology, Cell Proliferation and Viability, Immunology, Stem Cells
- Molecular Family
- Growth Factors, Cytokines/Chemokines
- Antigen References
-
1. Kawasaki ES, et al. 1985. Science 230:291.
2. Wei S, et al. 2010. J. Leukoc. Biol. 88:495.
3. Hodge JM, et al. 2011. PLoS One 6:e21462.
4. Morandi A, et al. 2011. PLoS One 6:e27450.
5. Erblich B, et al. 2011. PLoS One 6:e26317.
6. MacDonald KP, et al. 2010. Blood 116:3955. - Gene ID
- 1435 View all products for this Gene ID
- UniProt
- View information about M-CSF on UniProt.org