- Regulatory Status
- RUO
- Other Names
- Neuroregulin 1, Neu differentiation factor (NDF), Acetylcholine receptor-inducing activity chick homolog of (ARIA), glial growth factor, Heregulin alpha, Sensory and motor neuron derived factor.
-
Human NRG1α induces the proliferation of human mammary epithelium MCF7 cells.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 765704 | 25 µg | $147.00 | |||
Neuregulin 1 (NRG1) was initially identified in the conditioned medium of a human breast tumor cell line as a protein of 45 kD, which induces the phosphorylation of a tyrosine kinase (ERBB2). The neuregulin family includes four members (NRG1-NRG4) that are encoded from four individual genes. Through differential promoter usage and splicing, these members generate 12 isoforms. The best characterized is neuregulin 1, which includes eight isoforms; all isoforms contain (from N-terminus to C-terminus) an immunoglobulin-like (Ig) domain, a growth factor-like domain (EGF), a transmembrane (TM), and a cytoplasmic domain. Alternative splicing in the EGF-like domain of neuregulin 1 result in α and β isoforms. The EGF-like domain is necessary and sufficient for neuregulin bioactivity. The EGFR family of receptors includes four members (ErbB1 through ErbB4), and neuregulins bind to the ErbB3 and ErbB4 receptors. Neuregulins are released from the membrane by proteolytic cleavage, and this process is required for the binding of the neuregulin derived fragments to ErbB receptors. The neuregulin1-β isoform is predominant in the central nervous system and participates in development, survival, and metabolism in neuron and glial cells. Neuregulin1-β is neuroprotective and attenuates inflammatory responses induced by ischemic stroke in rats, preventing macrophage and microglial infiltration and astrocytic activation. Also, neuregulin1-β blocks the induction of pro-inflammatory and stress genes provoked by ischemia. Neuregulins 1-4 are expressed in approximately 25% of breast cancer carcinomas and increase breast cancer cell proliferation, increase tumorigenesis, and promote invasive characteristics of cancer cells. In this sense, neuregulin1-β induces MMP-1 and MMP-9 in cancer cell lines.
Product Details
- Source
- Human NRG1α isoform, amino acids (Ser20-Lys241) (Accession# Q02297), with a C-terminal 6x His tag, was expressed in 293E.
- Molecular Mass
- The 228 amino acid recombinant protein has a predicted molecular mass of approximately 24.8 kD. The DTT-reduced and non-reduced protein migrates at approximately 45 kD by SDS-PAGE. The predicted N-terminal amino acid is Ser.
- Purity
- >95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS, pH 7.2.
- Endotoxin Level
- Less than 0.01 ng/µg cytokine as determined by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- ED50 = 5 - 30 ng/ml, as determined by induction of MCF-7 cell proliferation.
- Application
-
Bioassay
- Application Notes
-
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Growth factor
- Distribution
-
Gastric epithelial cells, breast cancer cells, fibroblasts, and Schwann cells.
- Function
- Neuregulin 1 plays a key role in embryonic development of the nervous system, heart, and mammary glands. Also regulates the constitutive color and melanocyte function in human skin, and might maintain cardiac structure and function in adults.
- Interaction
- Melanocytes, melanoma cells, epithelial cells, gastric epithelial cells, mesenchymal cells, and Schwann cells.
- Ligand/Receptor
- ErbB3-ErbB2 and ErbB3-ErbB4.
- Bioactivity
- Induces proliferation of human mammary epithelium MCF7 cells.
- Cell Type
- Embryonic Stem Cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, Neural Stem Cells
- Biology Area
- Cancer Biomarkers, Neuroscience, Stem Cells
- Molecular Family
- Cytokines/Chemokines, Growth Factors
- Antigen References
-
1. Holmes WE, et al. 1992. Sciences 256:1205.
2. Corfas G, et al. 1993. Proc. Natl. Acad. Sci. USA 90:1624.
3. Tan W, et al. 2007. J. Biol. Chem. 282:24343.
4. Buac K, et al. 2009. Pigment Cell Melanoma Res. 22:773.
5. Shamir A and Buonanno A. 2010. J. Neurochem. 113:1163.
6. Mei L and Xiong WC. 2008. Nat. Rev. Neurosci. 9:437.
7. Luo X, et al. 2011. J. Biol. Chem. 286:23967.
8. Zhang K, et al. 2013. Pigment Cell Melanoma Res. 26:408. - Gene ID
- 3084 View all products for this Gene ID
- UniProt
- View information about NRG1alpha on UniProt.org