- Regulatory Status
- RUO
- Other Names
- Platelet Derived Growth Factor Receptor Alpha, PDGFR2, CD140a, CD140 Antigen-Like Family Member A
-
Recombinant human PDGFRα inhibits the proliferation of NIH3T3 mouse embryonic fibroblast cells in the presence of the human PDGF-AA in a dose-dependent manner with ED50 of 0.4 – 2.4 µg/mL. -
Stability Testing for Recombinant Human PDGFRα Chimera. Recombinant human PDGFRα Chimera was aliquoted in PBS at 0.2 mg/mL and one aliquot was kept at 4°C (Control), and another was frozen and thawed four times (4x Freeze/Thaw). After this procedure, the samples were tested for their ability to inhibit the proliferation of NIH3T3 in the presence of the human PDGF-AA in a dose-dependent manner. The ED50 for this effect is 0.4 – 2.4 µg/mL
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 569904 | 25 µg | $390.00 | |||
| 569906 | 100 µg | $820.00 | |||
The Platelet-derived growth factor (PDGF) family comprises four different polypeptide chains encoded by four genes. The four PDGF chains assemble into disulfide-bonded dimers via homo- or heterodimerization, and five different dimeric isoforms have been described so far; PDGF-AA, PDGF-AB, PDGF-BB, PDGF-CC, and PDGF-DD. PDGF is a major mitogen for fibroblasts, smooth muscle cells, and others. They exert their functions by interacting with two related receptor tyrosine kinases, PDGF receptor α (PDGFRα) and β, and the PDGFRα as a universal receptor. All the currently described PDGF receptor-binding components, e.g. phospholipase C-gamma, members of the src family of cytoplasmic tyrosine kinases, the rasGTPase activating protein and p85, the regulatory subunit of phosphatidylinositol 3' kinase, contain a conserved src homology 2-domain, through which the association with the receptor takes place. The receptor-binding components either possess an intrinsic enzymatic activity or function as adaptors, which may be complex with catalytically active components. PDGFRα plays a key role in many diseases through the above signal transduction.
Product Details
- Source
- Human PDGFRα, amino acid (Gln24-Glu524) (Accession: #P16234.1), with a linker (IEGRMD) and a C-terminal human IgG (Pro100-Lys330), was expressed in CHO cells.
- Molecular Mass
- The 738 amino acid recombinant protein has a predicted molecular mass of approximately 82.8 kD. The DTT-reduced and non-reduced protein shall migrate at approximately 85 -120 kD and 140 – 240 kD by SDS-PAGE, respectively. The predicted N-terminal amino acid is Gln.
- Purity
- > 95%, as determined by Coomassie-stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS, pH 7.2
- Concentration
- 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Human PDGFRα inhibits the proliferation of NIH3T3 mouse embryonic fibroblast cells in the presence of the human PDGF-AA in a dose-dependent manner with ED50 of 0.4 – 2.4 µg/mL.
- Application
-
Bioassay
- Application Notes
-
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Dimer
- Distribution
-
It is present in a variety of mesenchymal tissues such as the lungs, heart, intestines, and skin.
- Function
- The binding of its cognate ligands results in the activation of multiple signaling cascades such as MAPK, STAT, and PI3K pathways; stimulates cell proliferation and regulates cell migration and chemotaxis in wound healing
- Interaction
- Mesenchymal cells, Oligodendrocyte
- Ligand/Receptor
- Human PDGFAA, PDGFAB, PDGFBB, PDGFCC and PDGFDD
- Bioactivity
- Human PDGFRα inhibits the proliferation of NIH3T3 mouse embryonic fibroblast cells in the presence of the human PDGF-AA in a dose-dependent manner.
- Cell Type
- Embryonic Stem Cells, Mesenchymal cells, Mesenchymal Stem Cells, Neural Stem Cells
- Biology Area
- Angiogenesis, Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
- Claesson-Welsh L. 1994. Prog Growth Factor Res. 5:37-54.
- Boström H, et al. 1996. Cell. 85:863-73.
- Heldin CH, et al. 1999. Physiol Rev. 79:1283-316.
- Fredriksson L, et al. 2004. Cytokine Growth Factor Rev. 15:197-204.
- Andrae J, et al. 2008. Genes Dev. 22:1276-312.
- Yao L, et al. 2022. Cell Rep. 40:111192.
- Vantler M, et al. 2006. FEBS Lett. 580:6769-76.
- Vassbotn FS, et al. 1994. J Biol Chem.269:13874-9.
- Li R, et al. 2018. Elife.7:e36865.
- Gene ID
- 5156 View all products for this Gene ID
- UniProt
- View information about PDGFRa on UniProt.org