- Regulatory Status
- RUO
- Other Names
- CD170, OBBP2, CD33L2, OB-BP2, SIGLEC-5
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 557204 | 25 µg | $218.00 | |||
| 557206 | 100 µg | $546.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
Siglec proteins (sialic acid binding Ig-like lectins) are type I membrane proteins with an extracellular region which contains a sialic acid binding V-set Ig-like domain at the N-terminus, followed by varying numbers of C2-set Ig domains. In the cytosolic domain, most siglecs contain a combination of tyrosine motifs that include immunoreceptor tyrosine-based inhibitory motifs (ITIMs) along with ITIM-like, Grb2-binding, and Fyn kinase sites. Siglecs are widely expressed on hematopoietic cells, often in a cell type specific manner. Their ligands, sialic acids, are negatively charged monosaccharides found on cell surface glycoproteins and glycolipids. Studies suggest that Siglecs may participate in cell-to-cell interactions or can act as receptors during the entry of viral or bacterial pathogens. In addition, the presence of ITIMs indicate that these molecules play a role in the suppression of immunoreceptor signaling. Siglecs can be classified into two subgroups with Siglec 1, 2, and 4 as one group and a Siglec 3/CD33-related subgroup (Siglec 3 and Siglec 5 - 14) as the second. Siglec 5, a human CD33-related Siglec, selectively regulates early recruitment of neutrophils in the lung during lipopolysaccharide-induced acute lung inflammation.
Product Details
- Source
- Human Siglec 5, amino acids (Glu17-Thr434) (Accession# NP_003821), was expressed in CHO cells with a C-terminal human IgG1 Fc tag.
- Molecular Mass
- This 652 amino acid recombinant protein has a predicted molecular mass of approximately 72.7 kD. The protein migrates at approximately 120 kD in DTT-reduced conditions and approximately 250 kD in non-reduced conditions by SDS-PAGE. The predicted N-terminal amino acid is Glu.
- Purity
- >95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in 20 mM Tris, 150 mM NaCl, and pH 7.4.
- Endotoxin Level
- Less than 1.0 EU per µg of protein as determined by the LAL method.
- Concentration
- 25 µg size is bottled at 100 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Recombinant human Siglec 5 is able to agglutinate human red blood cells with an ED50 of 0.625 - 2.5 µg/mL.
- Application
-
Bioassay
- Application Notes
-
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Homodimer.
- Distribution
-
Human Siglec 5 is expressed by neutrophils, monocytes, macrophages, basophils, CD34+ cells, mast cells, and B lymphocytes.
- Function
- Siglec 5 regulates neutrophil recruitment. Siglec 5's negative signaling regulates the LPS-induced production of TNF-α and IL-6 by macrophages and is upregulated by a variety of TLR agonists.
- Interaction
- Red blood cells.
- Ligand/Receptor
- α2,3- and α2,6-linked disialic acid.
- Bioactivity
- Human Siglec 5 is able to bind and agglutinate human red blood cells.
- Cell Type
- Hematopoietic stem and progenitors
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Stem Cells
- Molecular Family
- Adhesion Molecules, CD Molecules, Siglec Molecules
- Antigen References
-
1. McMillan SJ, et al. 2013. Blood 121:2084.
2. Bax M, et al. 2010. Ann. Rheum. Dis. 69:42.
3. Angata T and Varki A. 2000. J. Biol. Chem. 275:22127.
4. Zhang JQ, et al. 2004. Eur. J. Immunol. 34:1175.
5. Yu Z, et al. 2001. Biochem. J. 353:483.
6. Yokoi H, et al. 2006. Allergy 61:769. - Gene ID
- 8778 View all products for this Gene ID
- UniProt
- View information about Siglec 5 on UniProt.org