- Regulatory Status
- RUO
- Other Names
- Thymic stromal lymphopoietin (TSLP)
-
Baf3-hIL7a/hTSLPR cell proliferation induced by human TSLP.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 582402 | 10 µg | $218.00 | |||
| 582404 | 25 µg | $405.00 | |||
| 582406 | 100 µg | $1155.00 | |||
| 582408 | 500 µg | $2578.00 | |||
TSLP was initially identified in conditioned medium from a mouse thymic stromal cell line. Human and mouse TSLP are only 43% identical at the amino acid level, and TSLP, like IL-7, exhibits species-specific functions and may function differently in humans and mice. TSLP binds to a heterodimeric receptor constituted by IL-7Ra and TSLPR. TSLPR has low affinity for TSLP, but in combination with IL-7Ra generates a high affinity binding site. TSLP has been associated with the development of allergic disorders in humans, such as asthma, atopic dermatitis, and food allergies. The susceptibility to allergic diseases has been associated to basophil induction by TSLP, and Th2 cytokine production induced by TSLP in mast cells. In addition, TSLP has been associated to the development of Tregs, and blocking TSLPR leads to delayed functional maturation of thymic Tregs. TSLPR has been detected in Tregs from allergic asthmatic patients. TSLP directly activates STAT5 in Tregs and suppresses its suppressive activities; additionally, it inhibits IL-10 production. Human TSLP induces the release of T cell-attracting chemokines (CCL17 and CCL22) from monocytes and enhances the T cell stimulatory capacity of the CD11c+ subset of dendritic cells. TSLP induces proliferation of naïve T cells. In addition, it can drive Th2 differentiation via the induction of IL-4 production, and drives Th2 cytokine-mediated inflammation by acting on bone marrow-resident progenitors, which promotes basophil hematopoiesis. TSLP is upregulated in epithelial cells by IL-1α, TNFα, and Th2 cytokines.
Product Details
- Source
- Human TSLP, amino acids Met-Tyr29-Gln159 (Accession# AF338732), was expressed in E. coli.
- Molecular Mass
- The 132 amino acid recombinant protein has a predicted molecular mass of approximately 15 kD. The DTT-reduced protein migrates at approximately 17 kD and non-reduced protein at approximately 18 kD by SDS-PAGE. The N-terminal amino acid is Met.
- Purity
- >98%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in 20 mM NaH2PO4, pH 7.2, 0.15 M NaCl.
- Endotoxin Level
- Less than 0.01 ng per µg cytokine as determined by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- ED50 = 0.4 - 2.4 ng/ml, as determined by the dose dependent stimulation of mouse BaF3-human IL-7a and human TSLPR cotransfectant cell proliferation.
- Application
-
Bioassay
- Application Notes
-
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Cytokine
- Distribution
-
Epithelial cells, mast cells, basophils, epidermal keratinocytes in atopic dermatitis, synovial fibroblast in arthritis rheumatoid, human airway smooth muscle cells
- Function
- Induces proliferation of naïve T cells, the release of T cell-attracting chemokines (CCL17 and CCL22) from monocytes, and the T cell stimulatory capacity of CD11c+ subset of dendritic cells; drives Th2 differentiation, drives Th2 cytokine-mediated inflammation to promote basophil hematopoiesis
- Interaction
- Monocyte, dendritic cells, activated human basophils, activated human T cells, Langerhans cells, B cells, mast cells, activated pulmonary Tregs, NKT cells
- Ligand/Receptor
- Heterodimeric receptor complex composed of the IL-7 receptor α chain (IL-7Rα) and the TSLP receptor (TSLPR or CRLF2)
- Biology Area
- Cell Biology, Immunology
- Molecular Family
- Cytokines/Chemokines, Growth Factors
- Antigen References
-
1. Levin SD, et al. 1999. J. Immunol. 162:677.
2. Reche PA, et al. 2001. J. Immunol. 167:336.
3. Omori M and Steven Ziegler. 2007. J. Immunol. 178:1396.
4. Rochman I, et al. 2007. J. Immunol. 178:6720.
5. Mazzuchelli R, et al. 2008. Blood 112:3283.
6. Siracusa MC, et al. 2011. Nature 477:229.
7. Nguyen KD, et al. 2010. Allergy Asthma Clin. Immunol. 6:4. - Gene ID
- 85480 View all products for this Gene ID
- UniProt
- View information about TSLP on UniProt.org