- Regulatory Status
- RUO
- Other Names
- B-Lymphocyte activation antigen B7.1, CD80, Ly53, TSA1, CD28 ligand (CD28L), MIC17
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Immobilized mouse B7.1 induces proliferation of human T cells in the presence of anti-human CD3. The ED50 is 1 - 10 µg/mL. In addition, mouse B7.1 induces IL-2 production on activated T cells. Results may vary depending on human donors.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 555404 | 25 µg | $147.00 | |||
| 555406 | 100 µg | $347.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
The B7 family of proteins are structurally related, cell membrane-associated molecules that regulate immune responses by delivering costimulatory or coinhibitory signals via their ligands. So far, eight family members have been identified, including B7.1, B7.2, B7-H1, B7-H2, B7-H4, B7-H6, PD-L1, and PD-L2. These proteins are mainly expressed by activated antigen presenting cells (APCs) and when paired with either CD28 or CTLA-4 on a T lymphocyte, they induce an increase or decrease in the downstream signaling activity of the MHC-TCR interaction between APCs and T cells. B7.1 has been shown to bind both CTLA-4 and CD28; however, it has a 20-fold higher affinity to CTLA-4 than to CD28. Despite their structural similarities, the individual contribution of B7.1 and B7.2 to the development of pathogenic T cells in autoimmune diseases and protective T cells in infectious diseases is markedly distinct. Besides regulating T cell immune responses, B7 also plays a role in anti-tumor immunity. This is confirmed by demostrating that cytotoxic T cells were able to eradicate tumor cells transfected with B7.1 and B7.2. After activation, follicular lymphoma was shown to upregulate B7.1 and other costimulatory and adhesion molecules, therefore increasing APC activity and amplifying primed T cell responses. Homozygous null B7.1/B7.2 mice fail to generate antigen specific IgG1 and IgG2a responses, suggesting that B7 proteins are important in Ig immune responses. Mouse and human B7.1 share 44% amino acid identity.
Product Details
- Source
- Mouse B7.1, amino acids (Asp37-Lys245) (Accession# NP_033985.3), was expressed in CHO cells with a C-terminal His-Fc tag and a linker sequence.
- Molecular Mass
- The 449 amino acid recombinant protein has a predicted molecular mass of approximately 51 kD. The protein migrates at approximately 72 kD in DTT-reducing conditions and at approximately 150 kD in non-reducing conditions by SDS-PAGE. The predicted N-terminal amino acid is Asp.
- Purity
- >95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS.
- Endotoxin Level
- Less than 1.0 EU per µg of protein as determine by the LAL method.
- Concentration
- 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Immobilized mouse B7.1 induces proliferation of human T cells in the presence of anti-human CD3. The ED50 is 1 - 10 µg/mL. In addition, mouse B7.1 induces IL-2 production on activated T cells. Results may vary depending on human donors.
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
- Product Citations
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Antigen Details
- Structure
- Homodimer
- Distribution
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Activated B cells, monocytes, macrophages, dendritic cells
- Function
- Plays a role in T cell activation; CD80 and CD86 play an important role in T cell priming.
- Interaction
- T cells
- Ligand/Receptor
- CD28, CTLA-4
- Bioactivity
- Immobilized mouse B7.1, in conjuction with anti-CD3, is able to induce human T cell proliferation and IL-2 secretion.
- Biology Area
- Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors, Soluble Receptors
- Antigen References
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1. Freeman GJ, et al. 1993. Science 262:909.
2. Azuma M, et al. 1993. Nature 366:76.
3. Gimmi CD, et al. 1991. Proc. Natl. Acad. Sci. USA 88:6575.
4. Linsley PS, et al. 1991 J. Exp. Med. 173:721.
5. Greaves P and Gribben JG, 2013. Blood. 121: 734.
6. Czuczman MS, et al. 2005. J. Clin. Oncol. 23:4390. - Gene ID
- 12519 View all products for this Gene ID
- UniProt
- View information about B7.1 on UniProt.org