- Regulatory Status
- RUO
- Other Names
- Monocyte chemotactic protein 2 (MCP-2)
-
Recombinant mouse CCL8 (MCP-2) chemoattracts Baf3-mCCR5 transfectants in a dose-dependent manner. The ED50 for this effect is 0.2 - 1.2 µg/mL.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 581702 | 10 µg | $218.00 | |||
| 581704 | 25 µg | $405.00 | |||
| 581706 | 100 µg | $686.00 | |||
| 581708 | 500 µg | $2051.00 | |||
CCL8, also known as MCP-2, is a beta chemokine initially isolated from human MG-63 osteosarcoma cells stimulated with IL-1β. CCL8 (MCP-2) has structural and functional similarity with MCP-1 and MCP-3, and it is often co-induced with those chemokines in mononuclear cells. IFNγ is a strong inducer of CCL8 in monocytes and fibroblasts. CCL8 can be processed and secreted as a truncated chemokine CCL8 (6-75). The truncated isoform does not chemoattract monocytes, but it binds to the CCR2 receptor and induces its internalization; as a result, CCL8 inhibits MCP-1 (CCL2) and MCP-2 (CCL8) ERK signaling and antagonizes the chemotactic activity of several CCR2 ligands (MCP-1/CCL2, MCP-2/CCL8, MCP-3/CCL7). CCL8 is highly expressed in the skin, and it serves as an agonist for the chemokine receptor CCR8, but not for CCR2. Therefore, it has been speculated that the CCL8-CCR8 interaction is a crucial regulator of Th2 cell homing, which drives IL-5-mediated chronic allergic inflammation. In addition, CCL8 binds CCR5 receptor with high affinity and inhibits the replication of human immunodeficiency virus type 1. CCL8 gene expression (by splenic stromal cells) is potentially relevant to the regulation of dendritic cell (DC) development. In addition, CCL8 expression is induced in splenic stromal cells during L. donovani infection and, in conjuction with CXCL12, hematopoietic stem and progenitor cells (HSPCs) are chemoattracted and potentially differentiated into regulatory DCs. Finally, CCL8 is a potential biomarker for the diagnosis of graft-versus-host diseases, tuberculosis, and sepsis.
Product Details
- Source
- Mouse CCL8, amino acids Gly24-Pro97 (Accession# NM_021443.3) was expressed in E. coli.
- Molecular Mass
- The 74 amino acid recombinant protein has a predicted molecular mass of approximately 8.48 kD. The DTT-reduced protein migrates at approximately 11 kDa and non-reduced protein migrates at approximately 12 kD by SDS-PAGE. The N-terminal amino acid is Gly.
- Purity
- >98%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS.
- Endotoxin Level
- Less than 0.01 ng per µg cytokine as determined by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Recombinant mouse CCL8 (MCP-2) chemoattracts Baf3-mCCR5 transfectants in a dose-dependent manner. The ED50 for this effect is 0.2 - 1.2 µg/mL.
- Application
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Bioassay
- Application Notes
-
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Chemokine
- Distribution
-
Fibroblast, endothelial cells, monocytes, macrophages, and skeletal muscle cells.
- Function
- CCL8 chemoattracts monocytes, T cells, NK cells, mast cells, eosinophils, and basophils. CCL8 is induced by IL-1β, IFNβ, IFNγ, dsRNA, virus, ConA, and anti-CD2. The induction of CCL8 by IFNγ synergizes with the TLR ligands peptidoglycan (TLR2), dsRNA (TLR3), and LPS (TLR4).
- Interaction
- Monocytes, T cells, NK cells, mast cells, eosinophils, and basophils.
- Ligand/Receptor
- CCR1 (CD191), CCR2 (CD192), CCR3 (CD193), and CCR5 (CD195).
- Biology Area
- Cell Biology, Neuroinflammation, Neuroscience, Signal Transduction
- Molecular Family
- Cytokines/Chemokines
- Antigen References
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1. Van Damme J, et al. 1992. J. Exp. Med. 176:59.
2. Proost P, et al. 1998. J. Immunol. 160:4034.
3. Dean RA, et al. 2008. Blood 112: 3455.
4. Hori T, et al. 2008. Blood 111:4403.
5. Rom S, et al. 2010. FASEB J. 24:2292.
6. Struyf S, et al. 2009. Eur. J. Immunol. 39:843.
7. Islam SA, et al. 2011. Nat. Immunol. 12:167. - Gene ID
- 20307 View all products for this Gene ID
- UniProt
- View information about CCL8 on UniProt.org