- Regulatory Status
- RUO
- Other Names
- PVR, PVS, Nectin-like molecule-5, Nectin-like protein 5, NECL5, NECL-5, Poliovirus receptor
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When mouse CD155-Fc Chimera is immobilized at 2.0 µg/mL, biotinylated mouse TIGIT-Fc Chimera binds with an EC50 of 10 - 40 ng/mL. HRP Avidin (Cat. No. 405103) was used to detect the binding. -
Stability testing for mouse CD155-Fc Chimera. Recombinant mouse CD155-Fc Chimera was aliquoted in PBS, pH 7.2 at 0.2 mg/mL. One aliquot was frozen and thawed four times (4x Freeze/Thaw), and compared to a control kept at 4°C (Control). The samples were tested in a binding assay with biotinylated mouse TIGIT-Fc Chimera.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 786602 | 10 µg | $112.00 | |||
| 786604 | 25 µg | $229.00 | |||
| 786606 | 100 µg | $675.00 | |||
| 786608 | 500 µg | $1582.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CD155, also known as TAGE4 or NECL-5 (nectin-like molecule-5), was originally described as a poliovirus entry receptor (PVR) more than 30 years ago. CD155 is a 70 kDa type I transmembrane glycoprotein that belongs to the nectin-related family of adhesion molcules within the immunoglobulin superfamily, defined by the presence of immunoglobulin domains V, a C1-like domain and a C2 domain in the extracellular region. CD155 binds several molecules including Vitronectin, Nectin-3, DNAM-1/CD226, CD96, and TIGIT. All bindings are mediated by the V-type domain and alternative splicing within this domain can modulate ligand binding. CD155 is highly expressed on tumor cells of epithelial and neuronal origin. It's enhanced expression in these tumors contributes to the loss of contact inhibition leading to increased migration, however, tumor cell recognition and killing by NK cells are also enhanced via DNAM-1 or CD96 expressing NK cells. CD155 normally functions in the establishment of intercellular adherens junctions between epithelial cells. CD155 mediates NK cell adhesion and triggers NK cell effector functions by binding to CD96 and CD226. These interactions accumulates at the cell-cell contact site, leading to the formation of a mature immunological synapse between NK cell and target cells.
Product Details
- Source
- Mouse CD155, amino acid Asp29 - Leu348 (Accession # NM_027514.1), with a C-terminal hIgG1 Fc tag was expressed in 293E cells.
- Molecular Mass
- The 559 amino acid recombinant protein has a predicted molecular mass of approximately 61.7 kD. The DTT-reduced protein migrates at approximately 80 kD and non-reduced protein migrates at approximately 160 kD by SDS-PAGE. The predicted N-terminal amino acid is His.
- Purity
- > 95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS, pH 7.2.
- Endotoxin Level
- Less than 0.01 ng per µg cytokine as determined by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- When mouse CD155-Fc Chimera is coated at 2.0 μg/mL (100 μL/well), biotinylated mouse TIGIT-Fc Chimera binds with an EC50 of 10 - 40 ng/mL. HRP Avidin (Cat. No. 405103) was used to detect the binding.
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
- Product Citations
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Antigen Details
- Structure
- Disulfide-linked homodimer
- Distribution
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CD155 is highly expressed on immature thymocytes, lymph node dendritic cells, and tumor cells of epithelial and neuronal origin. It is preferentially expressed on Th17 cells.
- Function
- Mediates NK cell adhesion and triggers NK cell effector functions.
- Interaction
- NK cell, T cell
- Ligand/Receptor
- Vitronectin, Nectin-3, DNAM-1/CD226, CD96, and TIGIT.
- Bioactivity
- Measured by its ability to bind biotinylated mouse TIGIT a functional ELISA.
- Cell Type
- Dendritic cells, Th17, Thymocytes
- Biology Area
- Adaptive Immunity, Cell Adhesion, Cell Motility/Cytoskeleton/Structure, Neuroscience
- Molecular Family
- Adhesion Molecules, Soluble Receptors
- Antigen References
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- He Y, et al. 2000. Proc. Natl. Acad. Sci. USA. 97:79-84.
- Bottino C, et al. 2003. J. Exp. Med. 198:557-67.
- Fuchs A, et al. 2004. J. Immun. 172: 3994-3998.
- Reymond N, et al. 2004. J. Exp. Med. 199:1331-41.
- Koike S, et al. 1990. EMBO J. 9: 3217-3224.
- Mendelsohn CL, et al. 1989. Cell 56:855-65.
- Gene ID
- 52118 View all products for this Gene ID
- UniProt
- View information about CD155 on UniProt.org