- Regulatory Status
- RUO
- Other Names
- Delta1, Delta-1, Delta-like protein 1, Dll1, DLL-1
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Recombinant Mouse DLL1 enhances BMP9 induced alkaline phosphatase production in C3H10T1/2 cells with ED50 of 1.5 – 6.0 µg/mL. -
Stability testing for Mouse DLL1. Mouse DLL1 was aliquoted in PBS, pH 7.2 at 0.2 mg/mL. One aliquot was frozen and thawed four times (4x Freeze/Thaws), and compared to a control kept at 4°C (Control). The samples were tested for their ability to enhance BMP9 induced alkaline phosphatase production in C3H10T1/2 cells.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 777902 | 10 µg | $94.00 | |||
| 777904 | 25 µg | $194.00 | |||
| 777906 | 100 µg | $581.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
DLL1 (Delta-like protein 1) is a type I membrane protein that belongs to the DSL (Delta/Serrate/Lag2) family of Notch ligands. It is required for embryonic development and maintenance of adult stem cells. There are five Notch ligands (DLL1, DLL3, DLL4, Jagged-1 and Jagged-2) and four Notch receptors (Notch-1 to Notch-4) in mammals. DLL1 is widely expressed and mouse DLL1 shares 91% and 95% amino acid sequence identity with human and rat DLL1 respectively. Interactions of Notch receptors with their ligands result in sequential proteolysis by ADAM (a disintegrin and metalloprotease) and then by presenilin/gamma secretase, resulting in the shedding of the extracellular domains and the generation of soluble ICD (intracellular domain) signaling fragments which translocate to the nucleus to interact with transcriptional factors. DLL1 undergoes proteolytic processing in its extracellular domain by ADAM10 in a similar manner to the Notch receptor, and its ICD may participate in bidirectional signaling. DLL1-induced Notch signaling regulates cell lineage, cell specification, cell patterning and morphogenesis through its effects on differentiation and proliferation. DLL1 plays an important roles in brain development at many different levels. During cerebellar development, DLL1 is required for the formation of the Bergmann glial layer and its morphological maturation. During neocortex development, DLL1-Notch signaling coordinates progenitor cell division and differentiation across radial and zonal boundaries.
Product Details
- Source
- Mouse DLL1, amino acid Ser22-Gln516 (Accession # Q61483), with a C-terminal 8His tag was expressed in CHO cells.
- Molecular Mass
- The 508 amino acid recombinant protein has a predicted molecular mass of approximately 55.2 kD. The DTT-reduced and non-reduced protein migrates at approximately 65 kD and 60 kD respectively by SDS-PAGE. The predicted N-terminal amino acid is Ser.
- Purity
- >95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS, pH 7.2
- Endotoxin Level
- Less than 0.1 EU per µg protein as determined by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- ED50 = 1.5 – 6.0 µg/mL as measured by its ability to enhance BMP9 induced alkaline phosphatase production in C3H10T1/2 cells.
- Application
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Bioassay
- Application Notes
-
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Distribution
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Expressed in heart and pancreas, with lower expression in brain and muscle and almost no expression in placenta, lung, liver and kidney.
- Function
- Transmembrane ligand protein of Notch receptors
- Ligand/Receptor
- Notch1, Notch2 and Notch3
- Bioactivity
- Enhance BMP9 induced alkaline phosphatase production in C3H10T1/2 cells.
- Cell Type
- Embryonic Stem Cells
- Biology Area
- Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Stem Cells
- Molecular Family
- Adhesion Molecules
- Antigen References
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- Bettenhausen B, et al. 1995. Development. 121:2407-2418
- Shimizu K, et al. 2000. Biochem Biophys Res Commun. 276:385-389
- Jaleco AC, et al. 2001. J Exp Med. 194:991-1001
- Wright GJ, et al. 2004. Development. 131:5659-5669
- Kawaguchi D, et al. 2008. Development. 135:3849-3858
- Adam MG, et al. 2013. Cir Res. 113:1206-1218
- Nelson BR, et al. 2013. J Neurosci. 33:9122-9139
- Hiraoka Y, et al. 2013. Mol Brain. 6:25
- Gene ID
- 13388 View all products for this Gene ID
- UniProt
- View information about DLL1 on UniProt.org