- Regulatory Status
- RUO
- Other Names
- Inducible costimulator, AILIM (activation inducible lymphocyte immunomediatory molecule) and CRP 1 (CD28 related protein 1)
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Immobilized recombinant mouse ICOS at 2 µg/mL binds recombinant mouse B7-H2-biotin in a dose-dependent manner. The ED50 for this effect is 0.08 - 0.48 µg/mL
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 599104 | 25 µg | $171.00 | |||
| 599106 | 100 µg | $370.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
Inducible costimulatory molecule (ICOS) is a member of CD28 superfamily protein. It has unique roles in T cell activation and differentiation, splenic germinal center formation, and immunoglobulin class switching. The expression of ICOS within the first 48 hours after T cell activation suggests that it may contribute to the differentiation of T cells during the primary response. ICOS binds to its ligand B7H, which is constitutively expressed at low levels on the surface of APCs and is upregulated by TNF-α or lipopolysaccharide. It was demonstrated that ICOS-B7H2 interaction plays an important role in the generation of CD4+ and CD8+ Tregs, and mediates the suppressive functions of CD4+ Tregs by regulating CTLA-4 expression on cell surfaces induced by allogeneic CD40-activated B cells. In the ICOS-KO mice, the T cell-mediated isotype switching is impaired while the IFN-gamma response is slightly affected; in addition, the ICOS-negative T cells do not accumulate in B cell follicles. Also, secondary, but not primary, clonal proliferation of antigen-specific B cells is impaired in ICOS-KO mice, and therefore the generation of secondary antibody-secreting cells is also impaired. These results suggest that this protein plays a pivotal role in T cell activation and Th1/Th2 differentiation.
Product Details
- Source
- Mouse ICOS, amino acids (Glu21-Leu142) (Accession # NP_059508.1), was exprssed in CHO cells with a human IgG1 Fc tag at the C-terminus.
- Molecular Mass
- The 360 amino acid recombinant protein with a C-terminal human IgG1 Fc tag has a predicted molecular mass of approximately 44 kD. The protein migrates as above 60 kD in SDS-PAGE in DTT-reducing condition and above 110 kD in non-reducing condition. The N-terminal amino acid is Glu.
- Purity
- >95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS.
- Endotoxin Level
- Less than 0.1 EU per µg of protein as determine by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Immobilized recombinant mouse ICOS at 2 µg/mL binds recombinant mouse B7-H2-biotin in a dose-dependent manner. The ED50 for this effect is 0.08 - 0.48 µg/mL
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Distribution
-
Activated T cells.
- Function
- ICOS regulates the production of IL-4 and IL-10, immunoglobulin-isotype class switching, the formation of germinal centers, and regulation of T cell proliferation. ICOS expression is influenced by TCR and CD28 signals. CD28 costimulation enhances ICOS expression, and ICOS upregulation is markedly reduced in the absence of B7-1 and B7-2.
- Interaction
- Antigen presenting cells, B cells.
- Ligand/Receptor
- ICOSL, CD275.
- Bioactivity
- Mouse ICOS is able to bind hB7-H2 .
- Cell Type
- B cells, Dendritic cells, Tregs
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Signal Transduction
- Molecular Family
- Immune Checkpoint Receptors, Soluble Receptors, TCRs
- Antigen References
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1. Mages HW, et al. 2000. Eur. J. Immunol. 30:1040.
2. Arimura Y, et al. 2002. Int. Immunol. 14:555.
3. Mahajan S, et al. 2007. Eur. J. Immunol. 37:1796.
4. Harada H, et al. 2003. J. Clin. Invest. 112:234.
5. Dong C, et al. 2001. Nature. 409:97.
6. McAdam AJ, et al. 2001. Nature. 409:102. - Gene ID
- 54167 View all products for this Gene ID
- UniProt
- View information about ICOS on UniProt.org