Pricing & Availability
Technical data sheet
- Regulatory Status
- RUO
- Other Names
- Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF)
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 714006 | 100 µg | $253.00 | |||
This product is not available for shipping outside of the United States.
Description
Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II expression by antigen presenting cells.
Product Details
- Source
- Mouse IFN-γ, amino acids His23-Cys155 (Accession # NM_008337), was expressed in E. coli.
- Molecular Mass
- The 134 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 15.7 kD. The predicted N-terminal amino acid is Met.
- Purity
- >98%, as determined by Coomassie stained SDS-PAGE and HPLC analysis.
- Formulation
- Lyophilized, carrier-free.
- Endotoxin Level
- Less than 0.1 ng per µg of protein.
- Storage & Handling
- Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 1.0 mg/ml. Do not vortex. It is recommended to further dilute in a buffer, such as 5% Trehalose, and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
- Activity
- ED50 is ≤ 0.2 ng/mL, corresponding to a specific activity ≥ 5 x 106 units/mg as determined by its ability to inhibit the proliferation of murine WEHI-279 cells.
- Application
-
Bioassay
- Product Citations
-
Antigen Details
- Structure
- Cytokine
- Distribution
-
CD8+ and CD4+ T cells, NK cells.
- Function
- Upregulated by IL-2, FGF-basic, EGF; downregulated by vitamin D3 or DMN; labile at pH2.
- Interaction
- T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts.
- Ligand/Receptor
- IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1).
- Bioactivity
- Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on antigen presenting cells.
- Cell Type
- Embryonic Stem Cells
- Biology Area
- Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Reynolds DS, et al. 1987. J. Immunol. 139:767.
2. Dey S, et al. 2010. Mol. Cell. Biol. 30:2181.
3. Sun T, et al. 2012. Proc. Natl. Acad. Sci. USA 109:E2117. - Gene ID
- 15978 View all products for this Gene ID
- UniProt
- View information about IFN-gamma on UniProt.org