- Regulatory Status
- RUO
- Other Names
- IL-15
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Mouse IL-15 induces the proliferation of CTLL2 mouse cells. The ED50 for this effect is 2.5 – 15 ng/mL. -
Mouse IL-15 induces the proliferation of HT-2 cells. The ED50 for this effect is 0.2 - 1.4 ng/mL.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 566301 | 2 µg | $89.00 | |||
| 566302 | 10 µg | $218.00 | |||
| 566304 | 100 µg | $909.00 | |||
This product is not available for shipping outside of the United States.
IL-15 was discovered in the supernatant of Simian kidney epithelial cell line CV-1/EBNA, as a soluble factor capable of supporting proliferation of the IL-2-dependent cell line, CTLL-2. IL-15 is a regulatory cytokine, and it is produced by dendritic cells, epithelial cells, fibroblasts, and monocytes. IL-15 plays an important role in immune response and shares many functions with IL-2. For example, it stimulates the proliferation of activated T cells, NK cells, and B cells, and induces immunoglobulin synthesis by B cells stimulated by anti-IgM or CD40 ligand. In addition, IL-15 promotes the development of dendritic cells and induces the production of proinflammatory cytokines from macrophages. IL-15 acts as a bridge between innate and adaptive immunity because of its diverse roles in the immune system.
IL-15 binds to heterotrimeric receptors composed of IL-15Rα, IL-15Rβ, and IL-15Rγc. IL-15 shares the receptor chains β and γc with IL-2. IL-15 is normally not secreted in soluble form but is instead held on the cell surface bound to a unique receptor, IL-15Rα, especially on dendritic cells. Cell-bound IL-15 is then presented in trans form to T cells and NK cells and is recognized by the γc receptor on these cells; such recognition maintains cell survival and intermittent proliferation.
Product Details
- Source
- Mouse IL-15, amino acids Asn49-Ser162 (Accession# NM_008357), was expressed in E. coli.
- Molecular Mass
- The 115 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 13,382 Da.
- Purity
- Purity is >95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- Lyophilized from a concentrated solution (1 mg/ml) with 10 mM NaH2PO4, 150 mM NaCL, pH 8.0.
- Endotoxin Level
- Less than 1 EU/µg cytokine as determined by the LAL method.
- Preparation
- For maximum results, quick spin vial prior to opening. Reconstitute in sterile water to a concentration of 0.1 mg/ml including a carrier protein such as 1% BSA or HSA or 10% FBS.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20°C to -70°C. Stock solutions can also be prepared at 50-100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1% BSA or HSA and stored in working aliquots at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
- Activity
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Mouse IL-15 induces the proliferation of CTLL2 mouse cells. The ED50 for this effect is 2.5 – 15 ng/mL.
Mouse IL-15 induces the proliferation of HT-2 cells. The ED50 for this effect is 0.2 - 1.4 ng/mL. - Application
-
Bioassay
- Application Notes
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This IL-15 protein is biologically active and can be used for in vitro assays.
- Product Citations
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Antigen Details
- Structure
- Cytokine
- Distribution
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IL-15 is expressed by DC, epithelial cells, fibroblasts, and monocytes.
- Function
- IL-15 stimulates the proliferation of activated T cells, NK cells, and B cells, and inducing immunoglobulin synthesis by B cells stimulated by anti-IgM or CD40 ligand. In addition, IL-15 promotes the development of dendritic cells, and induces the production of proinflammatory cytokines from macrophages.
- Ligand/Receptor
- IL-15Rα, IL-15Rβ and IL-15γc
- Cell Type
- Hematopoietic stem and progenitors
- Biology Area
- Immunology, Innate Immunity, Stem Cells
- Molecular Family
- Cytokines/Chemokines
- Antigen References
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1. Grabstein K, et al. 1994. Science 264:965.
2. Ma A, et al. 2006. Annu. Rev. Immunol. 24:657.
3. Meresse B, et al. 2004. Immunity 21:357.
4. Armitage RJ, et al. 1995. J. Immunol. 154:483.
5. Pulendran B, et al. 2004. Eur. J. Immunol. 34:66.
6. Bouchard A, et al. 2004. J. Leukoc. Biol. 76:162.
7. Ratthe C, et al. 2004. J. Leukoc. Biol. 75:893.
8. Feng T, et al. 2008. Cell. Immunol. 5:189.
9. Rubinstein MP, et al. 2006. P. Natl. Acad. Sci. USA 103:9166. - Gene ID
- 16168 View all products for this Gene ID
- UniProt
- View information about IL-15 on UniProt.org