- Regulatory Status
- RUO
- Other Names
- Il-1b, IL-1beta
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Recombinant Mouse IL-1β induces the proliferation of mouse helper lymphocyte D10.G4.1 cell line in a dose dependent manner. BioLegend’s protein was compared side-by-side to a competitor’s equivalent product.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 575102 | 10 µg | $206.00 | |||
| 575104 | 25 µg | $347.00 | |||
| 575106 | 100 µg | $909.00 | |||
| 575108 | 500 µg | $2461.00 | |||
Interleukin-1 is a key mediator of inflammation, with pleiotropic effects on several cells and signaling pathways. The activity defined as IL-1 reflects the function of 2 molecules, IL-1α and IL-1β. IL1A encodes IL-1α, which is cell-bound, and IL1B encodes IL-1β, a secreted cytokine (1). IL-1α and IL-1β are synthesized as 31-kD precursors and are processed by proteases to their mature 17-kD forms. IL-1β-converting enzyme cleaves the inactive IL-1β Precursor and ProIL-1α is processed by calpain (4). IL-1β is a tumor-promoting cytokine, and it enhances tumor metastasis and angiogenesis. IL-1β is able to facilitate tumor progression in murine models of lung cancer. Upregulation of metastasis and tumor angiogenesis by IL-1β has been associated with increased activity of matrix metalloproteinases and expression of the pro-angiogenic molecule hepatocyte growth factor (5).
Product Details
- Source
- Mouse IL-1β, amino acids Val118-Ser269 (Accession # NM_008361) was expressed in E. coli.
- Molecular Mass
- The 152 amino acid recombinant protein has a predicted molecular mass of 17,394 Da. The DTT-reduced and the non-reduced protein migrate at approximately 19kDa by SDS-PAGE. The N-terminal amino acid is Valine.
- Purity
- Purity is >98%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in 10mM NaH2PO4, 150mM NaCl, pH 7.2.
- Endotoxin Level
- Endotoxin level is <0.1 EU/µg (<0.01ng/µg) protein as determined by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Mouse IL-1β induces the proliferation of D10.G4.1 mouse helper lymphocytes. The ED50 = 2 - 10 pg/ml.
- Application
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Bioassay
- Recommended Usage
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Use when high specific biological activity is required.
- Application Notes
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This IL-1β protein is biologically active, and can be used for in vitro assays
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com. -
Application References
(PubMed link indicates BioLegend citation) -
- OÆSullivan BJ, et al. 2006. J. Immunol. 176:7278. PubMed
- Product Citations
-
Antigen Details
- Structure
- Cytokine
- Distribution
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IL-1β is produced by monocytes, macrophages, keratinocytes, stimulated B lymphocytes, fibroblasts, endothelial cells, and epithelial cells.
- Ligand/Receptor
- Type I and II IL-1 receptors (IL-1RI and IL-1RII)
- Cell Type
- Hematopoietic stem and progenitors
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Stem Cells
- Molecular Family
- Cytokines/Chemokines
- Antigen References
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1. Johnsen AK, et al. Arthritis Rheum 58:1947-1957 2008.
2. Brinster C and Shevach EM J. Leukoc. Biol. 84:480-487 2008.
3. O'Sullivan BJ, et al. J. Immunol. 176:7278-7287 2006.
4. Nazarenko I, et al. Neoplasia 10:549-562 2008.
5. Boost KA, et al. BMC Cancer 8:265 2008. - Gene ID
- 16176 View all products for this Gene ID
- UniProt
- View information about IL-1beta on UniProt.org