- Regulatory Status
- RUO
- Other Names
- Interleukin-2, T cell growth factor (TCGF), Eosinophil differentiation factor (EDF), Killer cell helper factor (KHF), Macrophage-activating factor for cytotoxicity I (MAF-C I), Thymocyte differentiation factor (TDF)
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 714604 | 20 µg | $253.00 | |||
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IL-2 was discovered through its function as a T cell growth factor (TCGF), and plays a pivotal role in immune responses against pathogenic infection. Recognition and binding of foreign antigens by T cell receptors stimulate both the secretion of IL-2 and the expression of IL-2Rs on the T cell surface. Subsequently, the IL-2/IL-2R interaction activates the intracellular Ras/Raf/MAPK, JAK/STAT, and PI3K/AKT signal pathways, and ultimately stimulates the growth, differentiation, and survival of the antigen-specific cytotoxic T cells. Human IL-2 acts on murine and human T cells, and its receptors are shared by others cytokines. IL-2Ra is an IL-2-specific receptor, IL-2Rb is shared with IL-15 and the gc is a common receptor shared by many cytokines including IL-2, IL-4, IL-7, IL-9, IL-15, and IL-21.
Product Details
- Source
- Mouse IL-2, amino acids Phe22-Gln169 (Accession# NM_008366), was expressed in E. coli.
- Molecular Mass
- The 148 amino acid recombinant protein has a predicted molecular mass of 17.2 kD. The predicted N-terminal amino acid is Phe.
- Purity
- >98%, as determined by Coomassie stained SDS-PAGE and HPLC analysis.
- Formulation
- Lyophilized, carrier-free.
- Endotoxin Level
- Less than 0.1 ng per µg of protein.
- Storage & Handling
- Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. It is recommended to further dilute in a buffer, such as 5% Trehalose, and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
- Activity
- ED50 is ≤ 0.2 ng/ml, corresponding to a specific activity of ≥ 5.0 x 106 units/mg as determined by the dose-dependent stimulation of the proliferation of murine CTLL-2 cells.
- Application
-
Bioassay
- Product Citations
-
Antigen Details
- Structure
- Cytokine
- Distribution
- Activated T cells
- Function
- Upregulated by NFAT; downregulated by TCF-8, CIF (colostrum inhibitory factor)
- Interaction
- T cells, B cells, NK cells, LAK cells, monocytes, macrophages, oligodendrocytes
- Ligand/Receptor
- High affinity heterotrimer of IL-2Rα/β/γ, intermediate affinity homodimer IL-2Rα (CD25; p55; Tac) and heterodimer IL-2Rβ (CD122)/γ; γ-subunit (CD132) in common with IL-4R, IL-7R, IL-13R, IL-15R .
- Bioactivity
- Proliferation of T lymphocytes, B cells, anti-inflammatory, hematopoiesis, tumor surveillance
- Cell Type
- Hematopoietic stem and progenitors, Embryonic Stem Cells
- Biology Area
- Stem Cells, Immunology, Cell Biology
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Detanico T, et al. 2011. J. Immunol. 187:82.
2. Malu DT, et al. 2011. J. Immunol. 186:6271.
3. Alcaide P, et al. 2012. J. Immunol. 188:1421.
4. Williams JW, et al. 2013. Am. J. Physiol. Lung Cell Mol. Physiol. 305:693. - Gene ID
- 16183 View all products for this Gene ID
- UniProt
- View information about IL-2 on UniProt.org