Interleukin-2, T cell growth factor (TCGF), Eosinophil differentiation factor (EDF), Killer cell helper factor (KHF), Macrophage-activating factor for cytotoxicity I (MAF-C I), Thymocyte differentiation factor (TDF)
HT-2 cell proliferation induced by mouse IL-2.
HT-2 cell proliferation induced by mouse IL-2.
Recombinant mouse IL-2 induces the proliferation of mouse HT-2 cell line in a dose dependent manner. BioLegend’s protein was compared side-by-side to a competitor’s equivalent product.
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Size
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575402
10 µg
$94.00
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575404
25 µg
$211.00
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575406
100 µg
$469.00
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575408
500 µg
$1465.00
Description
IL-2 is a potent lymphoid cell growth factor which exerts its biological activity primarily on T cells. Additionally, IL-2 has been found to stimulate growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendocytes.
Mouse IL-2, amino acids Ala21-Gln169 (Accession # NM_008366) was expressed in E. coli.
Molecular Mass
The 150 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 17,231 Da. The DTT-reduced protein and non-reduced protein migrates at approximately 18kDa by SDS-PAGE.
Purity
Purity is >98%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in 5 mM NaH2PO4, 5 mM citric acid, 350 mM NaCl, 1 mM TCEP, pH 4.0.
Endotoxin Level
Endotoxin level is <0.1 EU/µg (<0.01ng/µg) protein as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
ED50 = 0.2 - 1.0 ng/ml, corresponding to a specific activity of 1.0 - 5.0 x106 units/mg, as determined by the dose dependent stimulation of CTLL2 cell proliferation.
The specific activity of recombinant mouse IL-2 is approximately 9.07 x 104 IU/µg when compared against the Non WHO Reference Material for Murine Interleukin-2 (NIBSC code: 93/566) as determined by the dose dependent stimulation of CTLL2 cell proliferation.
ED50 = 0.4 - 2.0 ng/ml, corresponding to a specific activity of 0.5 - 2.5 x106 units/mg, as determined by the dose dependent stimulation of HT-2 cell proliferation. For more information on specific activity, please visit the Recombinant Protein Unit Conversions page.
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Additional Product Notes
Get a 50% discount on this product when purchased in our Activation Bundles. Restrictions apply. Learn more…
Application References
(PubMed link indicates BioLegend citation)
Detanico T, et al. 2011.J. Immunol. 187:82. PubMed
Malu DT, et al. 2011. J. Immunol. 186:6271. PubMed
Alcaide P, et al. 2012. J. Immunol. 188:1421. PubMed
Williams JW, et al. 2013. Am J Physiol Lung Cell Mol Physiol. 305:693. PubMed
Product Citations
Vella JL, et al. 2021. Life Sci Alliance. 4:. PubMed
Proliferation of T lymphocytes, B cells, anti-inflammatory, hematopoiesis, tumor surveillance
Cell Sources
T cells
Cell Targets
T cells, B cells, NK cells, LAK cells, monocytes, macrophages, oligodendrocytes
Receptors
High affinity heterotrimer of IL-2Rα/β/γ, intermediate affinity homodimer IL-2Rα (CD25; p55; Tac) and heterodimer IL-2Rβ (CD122)/γ; γ-subunit (CD132) in common with IL-4R, IL-7R, IL-13R, IL-15R
Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors
Biology Area
Cell Biology, Stem Cells
Molecular Family
Cytokines/Chemokines
Regulation
Upregulated by NFAT; downregulated by TCF-8, CIF (colostrum inhibitory factor)
