- Regulatory Status
- RUO
- Other Names
- Interleukin-22, IL-TIF
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 576202 | 10 µg | $229.00 | |||
| 576204 | 25 µg | $452.00 | |||
| 576206 | 100 µg | $1219.00 | |||
| 576208 | 500 µg | $3456.00 | |||
IL-22, a cytokine structurally related to IL10, was originally identified in the mouse as a gene induced by IL9 in T cells and mast cells (1). IL-22 was initially designated as ILTIF (IL10-related T cell-derived inducible factor). Mouse IL-TIF consists of 179 amino acids, including four cysteins, and has a predicted molecular weight of 20 KD. IL-22 shows 22% sequence identity with mouse IL-10 and belongs to a family of cytokines with limited homology to IL-10, namely IL-10, IL-19, IL-20, IL-24, IL-26, IL-28A, IL-28B, and IL-29 (the latter 3 also known as IFN-λ) (3). IL-22 biological activity is initiated by binding to a cell-surface complex composed of IL-22R1 and IL-10R2 receptor chains and further regulated by interactions with a soluble binding protein, IL-22BP, which shares sequence similarity with an extracellular region of IL-22R1 (sIL-22R1) (3). Both chains of the IL-22R complex belong to the class II CRF. Two types of IL-22-binding receptor have been discovered, a membrane-bound receptor and a soluble receptor, both encoded by different genes (4). IL-22 is produced by immune cells and acts on nonimmune cells to regulate local tissue inflammation. As a product of the recently identified T helper 17 lineage of CD4(+) effector lymphocytes, IL-22 plays a critical role in mucosal immunity as well as in dysregulated inflammation observed in autoimmune diseases (5).
Product Details
- Source
- Mouse IL-22, amino acids Leu34-Val179 (Accession # NM_016971) was expressed in E. coli.
- Molecular Mass
- The 147 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of approximately 16.8 kDa. The DTT-reduced protein migrates at approximately 15 kDa and the non-reduced protein migrates at approximately 13 kDa by SDS-PAGE.
- Purity
- Purity is >98%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in 10mM NaH2PO4, 150mM NaCl, pH 7.2.
- Endotoxin Level
- Endotoxin level is <0.1 EU/µg (<0.01ng/µg) protein as determined by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- The ED50 is 0.095-0.265 ng/ml, corresponding to a specific activity of 1.05-0.38 X107 units/mg, as determined by a dose dependent stimulation of human Colo205 cells in production of IL-10.
- Application
-
Bioassay
- Recommended Usage
-
Use when high specific biological activity is required.
- Application Notes
-
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
- Product Citations
-
Antigen Details
- Biology Area
- Cell Biology, Immunology, Stem Cells
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Dumoutier L, et al. 2000. J. Immun. 164:1814.
2. Xie MH, et al. 2000. J. Biol. Chem. 275:31335.
3. Jones BC, et al. 2008. Structure.
4. Kotenko SV, et al. 2001. J. Immun. 166:7096.
5. Chang C, et al. 2006. Cell Research 16:902. - Gene ID
- 50929 View all products for this Gene ID
- UniProt
- View information about IL-22 on UniProt.org