- Regulatory Status
- RUO
- Other Names
- Interleukin-22 receptor subunit alpha-1, IL-22 receptor subunit alpha-1, IL-22R subunit alpha-1, IL-22R alpha-1, IL-22Rα1, IL-22Ra1, Cytokine receptor class-II member 9, Cytokine receptor family 2 member 9, CRF2-9
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Mouse IL-22Rα1 inhibits the production of IL-10 in Colo 205 human adenocarcinoma cells induced by mouse IL-22. -
Stability testing for mouse IL-22Rα1. Mouse IL-22Rα1 was aliquoted in PBS at 0.2 mg/ml; one aliquot was kept at 4°C (control) and another was freeze-thawed four times (4x freeze-thaws). After this procedure, the samples were tested by its property to inhibit the production of IL-10 induced in Colo 205 human adenocarcinoma cells by recombinant mouse IL-22.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 774502 | 10 µg | $112.00 | |||
| 774504 | 25 µg | $183.00 | |||
| 774506 | 100 µg | $563.00 | |||
| 774508 | 500 µg | $1465.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
IL-22Rα1, also known as CRF2-9, is a type I transmembrane protein, belonging to type II cytokine receptor family. IL-22Rα1 can form receptor complexes with either IL-10Rβ (IL-10R2) or IL-20Rβ (IL-20R2) for different ligands. IL-22Rα1/IL-10Rβ is a receptor for IL-22, and IL-22Rα1/IL-20Rβ is responsive to IL-20 and IL-24. IL-22 initially binds to IL-22Rα1 with high affinity, then recruits IL-10Rβ to stabilize the ligand/receptor complex, activating the downstream signaling, including JAK/TYK2/STAT3 and MAPK pathway. IL-22 has high affinity binding for IL-22BP, and it is 20- to 1000-fold higher compared to its binding to the membrane bound IL-22Rα1, attenuating IL-22-induced response. Though IL-22 is secreted mainly by the lymphoid cells, IL-22Rα1 expression is restricted to epithelial cells, hepatocytes, stromal cells and acinar cells of the pancreas, and its expression is absent or extremely low on hematopoietic cells. The expression is also found in blood-brain barrier endothelial cells in multiple sclerosis lesions. Upon LPS stimulation, IL-22Rα1 mRNA is upregulated in the liver of mice. In addition, IL-22Rα1 expression can be upregulated by IFNγ in keratinocytes, causing increasing expression of anti-microbial proteins, such as β-Defensin 2, 3 and S100A7. IL-22Rα1/IL-22 signaling is involved in integrity of epithelia and host defenses against extracellular pathogens. IL-22Rα1/IL-24 inhibit endothelial cell migration and differentiation, and VEGF (vascular endothelial growth factor)-induced angiogenesis.
Product Details
- Source
- Mouse IL-22Rα1, amino acid (Thr18-Ala228) (Accession: # Q80XZ4.1), with a C-terminal 6His tag, was expressed in 293E cells.
- Molecular Mass
- The 217 amino acid recombinant protein has a predicted molecular mass of approximately 24.89 kD. The DTT-reduced and non-reduced protein migrates at approximately 36 kD by SDS-PAGE. The predicted N-terminal amino acid is Thr.
- Purity
- >98%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in 1x PBS.
- Endotoxin Level
- Less than 0.1 EU/µg cytokine as determined by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- The ED50 is 3-12 µg/ml, as determined by inhibition of mouse IL-22-induced IL-10 production on Colo 205 human adenocarcinoma cell (in the presence of 1 ng/ml of mouse IL-22 (Cat. No. 576202).
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Cytokine receptor
- Distribution
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IL-22Rα1 expression is restricted to epithelial cells, stromal cells, keratinocytes, and hepatocytes.
- Function
- IL-22Rα1/IL-22 signaling is involved in integrity of epithelia and host defenses against extracellular pathogens. IL-22Rα1/IL-24 inhibit endothelial cell migration and differentiation, and VEGF (vascular endothelial growth factor)-induced angiogenesis.
- Ligand/Receptor
- IL-22, IL-20, IL-24
- Biology Area
- Stem Cells, Immunology, Cell Biology
- Molecular Family
- Soluble Receptors, Cytokine/Chemokine Receptors
- Antigen References
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- Li J et al. 2004. Int. Immunopharmacol. 4:693.
- Dumoutier L et al. 2000. J. Immunol. 164:1814.
- Meng S et al. 2010. J. Mol. Cell. Biol. 2:223.
- Parrish-Novak J et al. 2002. J. Biol. Chem. 277:47517.
- Kotenko SV et al. 2001. J. Biol. Chem. 276:2725.
- Dumoutier L et al. 2009. J. Biol. Chem. 284:26377.
- Tachiiri A et al. 2003. Genes Immun. 4:153.
- Bleicher L et al. 2008. FEBS. Lett. 582:2985.
- Wolk K et al. 2004. Immunity. 21:241.
- Wolk K et al. 2006. Eur. J. Immunol. 36:1309.
- Ramesh R et al. 2003. Cancer. Res. 63:5105.
- Aujla SJ et al. 2008. Nat. Med. 14:275.
- De Luca AT et al. 2010. Mucosal. Immunol. 3:361.
- Gene ID
- 230828 View all products for this Gene ID
- UniProt
- View information about IL-22Ralpha1 on UniProt.org