- Regulatory Status
- RUO
- Other Names
- IL-36 receptor antagonist protein, IL-36RN, IL-1F5, IL-1HY1, IL-1 delta, IL-1L1, FIL1 delta
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Dose-dependent inhibition of mouse IL-36β-induced IL-6 secretion in 3T3-L1 cells.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 760804 | 25 µg | $382.00 | |||
| 760806 | 100 µg | $932.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
IL-36Ra belongs to the IL-1 cytokine family, as a receptor antagonist. Like other IL-36 cytokines, IL-36Ra does not contain caspase cleavage sites, but removal of N-terminal methionine is required for its full activity, which might be mediated by neutrophil elastase. IL-36Ra shares sequence homology with IL-Ra, another receptor antagonist of the IL-1 family. IL-36Ra inhibits IL-36α, IL-36β, IL-36γ signaling by preventing their binding to IL-1RL2/IL-36R and the recruitment of IL-1RAP co-receptor, leading to blockage of downstream NF-kB and MAP kinase activation. The mechanism of inhibition is similar to the one that is established between IL-1Ra and IL-1. IL-36 Ra is involved in innate immunity and anti-inflammatory responses. IL-36Ra attenuates IL-36 cytokine-induced pro-inflammatory cytokine production by bone marrow derived dendritic cells and antagonizes IL-36 cytokine-induced IFN-γ, IL-4, and IL-17 production in CD4+ T cells. In addition, the anti-inflammatory effect of IL-36Ra in brain is SIGIRR/TIR8-dependent. The deficiency of IL-36Ra is linked to psoriasis in humans. The deficit of IL-36Ra exacerbates skin inflammation in IL-36α transgenic mice, suggesting the importance of IL-36Ra as anti-inflammatory cytokine. The expression of IL-36Ra is induced by PMA and LPS in THP-1 cells and by Aspergillus fumigatus in human PBMCs.
Product Details
- Source
- Mouse IL-36Ra, amino acids Val2-Asp155 (Accession# BC132600) was expressed in E.coli.
- Molecular Mass
- The 154 amino acid recombinant protein has a predicted molecular mass of approximately 17 kD. The DTT-reduced and non-reduced protein migrate at approximately 17 kD by SDS-PAGE. The predicted N-terminal amino acid is Val.
- Purity
- >95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in 1x PBS.
- Endotoxin Level
- Less than 0.01 ng per µg cytokine as determined by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- The activity is determined by the dose-dependent inhibition of mouse IL-36β-induced IL-6 secretion in 3T3-L1 preadipocytes. The ED50 is 0.5 - 2 µg/ml in the presence of 3 ng/ml of mouse IL-36β.
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
- Product Citations
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Antigen Details
- Structure
- IL-1 cytokine family.
- Distribution
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Skin keratinocytes, detectable in splenocytes, and macrophage cell types.
- Function
- IL-36Ra is involved in anti-inflammatory response and prevents IL-36 cytokine-induced effect.
- Interaction
- Dendritic cells and lymphocytes.
- Ligand/Receptor
- IL-1RL2/IL-36R and IL-1RAcP.
- Biology Area
- Angiogenesis, Cell Biology, Immunology, Innate Immunity, Signal Transduction
- Molecular Family
- Cytokines/Chemokines
- Antigen References
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1. Garlanda C, et al. 2013. Immunity 39:1003.
2. Towne JE, et al. 2011. J. Biol. Chem. 286:42594.
3. Vigne S, et al. 2011. Blood 118:5813.
4. Costelloe C, et al. 2008. J. Neurochem. 105:1960.
5. Debets R, et al. 2001. J. Immunol. 167:1440.
6. Blumberg H, et al. 2007. J. Exp. Med. 204:2603.
7. Marrakchi S, et al. 2011. N. Engl. J. Med. 365:620.
8. Macleod T, et al. 2016. Sci. Rep. 6:24880.
9. Mulero JJ, et al. 1999. Biochem. Biophys. Res. Commun. 263:702. - Gene ID
- 54450 View all products for this Gene ID
- UniProt
- View information about IL-36Ra on UniProt.org