- Regulatory Status
- RUO
- Other Names
- Interleukin-4, B-cell stimulating factor-1 (BSF-1), Ia inducing factor (IaIF), Hodgkin's cell growth factor (HCGF), Mast cell growth factor-2 (MCGF-2), Macrophage fusion factor (MFF), T cell growth factor-2 (TCGF-2)
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CTLL-2 proliferation induced by mouse IL-4 -
ED50 = 0.6 - 3.6 ng/mL as measured by its ability to induce proliferation of HT-2 cell in a dose dependent manner. -
Recombinant mouse IL-4 induces the proliferation of mouse CTLL2 cell line in a dose dependent manner. BioLegend’s protein was compared side-by-side to a competitor’s equivalent product.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 574302 | 10 µg | $147.00 | |||
| 574304 | 25 µg | $253.00 | |||
| 574306 | 100 µg | $762.00 | |||
| 574308 | 500 µg | $2168.00 | |||
IL-4 is a member of the short four-helix bundle family of cytokines. IL-4 is produced by Th2 cells in response to antigen receptor engagement. Dendritic cells can prime naïve CD4+ T cell; nevertheless, it has been described that dendritic cell mediated priming is insufficient for the development of Th2 cell-dependent immunity. Basophils have been identified as a source of IL-4 in the initial naïve CD4+ T cell priming. These cells coexpress MHC class II and IL-4 following helminth infection. Therefore, murine basophils could present antigens to naïve T cells, thus promoting Th2 cell differentiation during allergic and anti-parasitic immune responses. In addition, it was previously known that upon cross-linkage of the high affinity receptor for immunoglobulin E, IL-4 is produced by NK cells, mast cells, and basophils. Cells of hematopoietic stem cell origin express the type I receptor for IL-4 (IL-4Ra, γc), and cells of non-hematopoietic stem cell origin express type II receptor (IL-4Ra, IL-13Ra1). Type I receptor complexes are active in regulating Th2 development. In contrast, the type II receptor complex is active in regulating cells that mediate airway hypersensitivity and mucus secretion.
Product Details
- Source
- Mouse IL-4, amino acids His23-Ser140 (Accession# NM_021283), was expressed in 293E cells using human IL-2 signal peptide.
- Molecular Mass
- The 118 (His23-Ser140) amino acid recombinant protein has a predicted molecular mass of 13.3 kD.
- Purity
- >95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS.
- Endotoxin Level
- Less than 0.01 ng per µg cytokine as determined by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
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ED50 = 0.3 - 1.8 ng/ml, corresponding to a specific activity of 0.55 - 3.3 x 106 units/mg, as determined by CTLL-2 cell proliferation induced by mouse IL-4 in a dose dependent manner.
ED50 = 0.6 - 3.6 ng/mL as measured by its ability to induce proliferation of HT-2 cell in a dose dependent manner.
The specific activity of recombinant mouse IL-4 is approximately 1.54 x 104 IU/µg when compared against the Non WHO Reference Material for Murine Interleukin-4 (NIBSC code: 91/656) as determined by the dose dependent stimluation of CTLL-2 cell proliferation.
For more information on specific activity, please visit the Recombinant Protein Unit Conversions page. - Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
- Additional Product Notes
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Get a 50% discount on this product when purchased in our Activation Bundles. Restrictions apply. Learn more…
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Application References
(PubMed link indicates BioLegend citation) - Product Citations
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Antigen Details
- Distribution
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IL-4 is produced by Th2 cells, NK T cells, mast cells, basophils, eosinophils, and macrophages.
- Interaction
- T cells, B cells, mast cells, monocytes, macrophages, fibroblast, and endothelial cells
- Ligand/Receptor
- Type I receptor complex (IL-4Rα, γc) and type II receptor complex (IL-4Rα, IL-13Rα1)
- Cell Type
- Embryonic Stem Cells, Hematopoietic stem and progenitors
- Biology Area
- Cell Biology, Immunology, Signal Transduction, Stem Cells
- Molecular Family
- Cytokines/Chemokines
- Antigen References
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1. Chen. L, et al. 2004. J. Immunol. 172:2059.
2. Maroof A, et al. 2006. Immunology 117:271.
3. Sharma P, et al. 2008. Immunity 29:551.
4. Heller NM, et al. 2008. Sci. Signal 1:ra17.
5. LaPorte SL, et al. 2008. Cell 132:259.
6. Perrigoue JG, et al. 2009. Nat. Immunol. 10:697.
7. Schneider E, et al. 2010. Eur. Cytokine Netw. 21:142.
8. Leavy O. 2011. Nat. Rev. Immunol. doi:10.1038/nri2908. - Gene ID
- 16189 View all products for this Gene ID
- UniProt
- View information about IL-4 on UniProt.org