- Regulatory Status
- RUO
- Other Names
- Interleukin-4 receptor subunit alpha, IL-4 receptor alpha chain, IL-4 receptor subunit alpha, IL-4R subunit alpha, IL-4R-alpha, CD124, CD124 antigen
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Recombinant mouse IL-4RA inhibits the HT-2 cell proliferation induced by mouse recombinant IL-4.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 766306 | 100 µg | $469.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
Mouse IL-4RA was initially cloned from mouse CTLL2 cells. It is a transmembrane glycoprotein type I. It possesses 810 amino acid; with an extracellular domain of 207 residues, a transmembrane domain of 24 residues, and the intracellular domain with 569. IL-4RA binds to IL-4 with high affinity, and a soluble receptor has been identified that possesses only the extracellular domain; the soluble receptor blocked the ability of IL-4 to induce CTLL cell proliferation. IL-4 binds to two heterodimeric IL-4 receptor complexes, the IL-4R type I (composed of the IL-4Rα and the common cytokine receptor γc chains) and II (composed of IL-4Rα and IL-13Rα1 chains) and it play a key role in Th2 cell differentiation. The IL-4R type II is shared with IL-13, and this interaction promotes airway inflammation and remodeling. The cytokines IL-4 and IL-13, acting through their heterodimeric IL-4 receptor complexes, play a crucial role in asthma pathogenesis; increased signaling via IL-4rα through STAT6 exacerbates allergic airway inflammation. Also, IL-4 enhances histamine release from peripheral blood basophils and mast cells in vitro and in vivo, and the sIL-4R inhibits histamine release. In addition, sIL-4R acts as antagonist of IL-4 in an infectious disease.
Product Details
- Source
- Mouse IL-4RA, amino acids (Ile26- Arg233) (Accession # NP_001008700) was expressed in 293E cells. The carboxy terminus contains SR-IEGRMD-hIgG1-Fc tag.
- Molecular Mass
- The 447 amino acid recombinant protein has a predicted molecular mass of approximately 51.2kD. The DTT-reduced and non-reduced protein migrate at approximately 70 kD and 140 kD respectively by SDS-PAGE. The predicted N-terminal amino acid is Ile.
- Purity
- > 95% by SDS-PAGE gel as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS at pH 7.2
- Endotoxin Level
- Less than 0.1 EU per µg cytokine as determined by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Mouse IL-4RA inhibits the proliferation of HT-2 mouse cells induced by mouse IL-4. The ED50 = 0.05 - 0.25 µg/ml.
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Soluble receptor
- Distribution
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T cells, B cells, mast cells, monocytes, macrophages, fibroblast, and endothelial cells
- Function
- Promotes Th2 differentiation, induction of Th2 associated cytokines, macrophage activation and TSLP production, regulation of IgE production, involved in asthma pathogenesis.
- Ligand/Receptor
- IL-4
- Bioactivity
- IL-4RA inhibits the proliferation of HT-2 cells induced by mouse IL-4.
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Signal Transduction, Transcription Factors
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors, Soluble Receptors
- Antigen References
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1. Mosley B, et al. 1989. Cell 59:335.
2. Gessner A, et al. 1994. Infect. Immun. 62:4112.
3. Hoffman RC, et al. 1995. Protein Science. 4:382.
4. Tachdjian R, et al. 2010. J. Allergy Clin. Immunol. 125:1128.
5. Noval Rivas M, et al. 2015. Immunity. 42(3):512-23.
6. Gour N and Wills-Karp M. 2015. Cytokines. 75:68.
7. Massoud AH. 2016. Nat Med. 22:1013. - Gene ID
- 16190 View all products for this Gene ID
- UniProt
- View information about IL-4RA on UniProt.org