- Regulatory Status
- RUO
- Other Names
- Interleukin-6, B cell stimulating factor-2 (BSF-2), Cytotoxic T cell differentiation factor (CDF), Hepatocyte stimulating factor (HSF), Hybridoma/plasmacytoma growth factor (HPGF)
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 715202 | 10 µg | $253.00 | |||
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IL-6 is a multifunctional cytokine that can regulate various immune and inflammatory responses. Several studies have suggested a crucial role for IL-6 in angiogenesis. The use of mice deficient in IL-6 (-/-) demonstrated a critical role for this protein in a mouse model of lung angiogenesis. IL-6 has been shown to cause proliferation and migration of systemic endothelial cells in culture. The classical responsiveness to IL-6 is governed by a receptor complex consisting of two membrane-bound subunits, an 80-kD cognate chain (IL-6R) and a ubiquitously expressed 130-kD β-chain receptor (gp130), which acts as the universal signal-transducing element for all IL-6 family cytokines. Alternatively, IL-6 regulation of leukocyte trafficking relies upon signaling via its soluble IL-6R (termed IL-6 trans-signaling). IL-6 plays a major role in regulating neutrophil clearance during acute peritoneal inflammation; as a result of specific downregulation of neutrophil-attracting chemokine (CXCL1/KC) production. IL-6 is a key factor that reciprocally regulates Th17 and FOXP3+ Treg differentiation by inhibition of TGF-β induced FOXP3 and induction of RORγt, a Th17 lineage-specific transcription factor.
Product Details
- Source
- Mouse IL-6, amino acids Phe25-Thr211 (Accession# NM_031168), was expressed in E. coli.
- Molecular Mass
- The 188 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 21.9 kD. The predicted N-terminal amino acid is Met.
- Purity
- >98%, as determined by Coomassie stained SDS-PAGE and HPLC analysis.
- Formulation
- Lyophilized from 0.1% TFA. Carrier-free.
- Endotoxin Level
- Less than 0.1 ng per µg of protein.
- Storage & Handling
- Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. It is recommended to further dilute in a buffer, such as 5% Trehalose, and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
- Activity
- ED50 is ≤ 0.02 ng/ml, corresponding to a specific activity of ≤ 5 x 107 units/mg as determined by the dose-dependent stimulation of the proliferation of murine 7TD1 cells.
- Application
-
Bioassay
- Product Citations
-
Antigen Details
- Distribution
-
IL-6 is released by activated T cells, B cells, monocytes, macrophages, fibroblasts, epithelial cells, and endothelial cells.
- Function
- Upregulated by IL-1, TNF, PDGF, IFN-β, TNF-α, NGF, IL-17; downregulated by glucocorticoids IL-4, TGF-β
- Interaction
- T cells, B cells, hepatocytes, cholinergic neurons
- Ligand/Receptor
- Heterodimer IL-6Rα (CD126)/IL-6Rβ (CD130, gp130)
- Bioactivity
- Hematopoiesis, antigen-specific immune responses, inflammatory reactions, acute phase reaction
- Cell Type
- Hematopoietic stem and progenitors
- Biology Area
- Angiogenesis, Cell Biology, Stem Cells
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. McClintock JE and Wagner EM. 2005. 99:861.
2. Murakami M. 1993. Science 260:1808.
3. Jones SA, et al. 2001. J. FASEB. 15:43.
4. Fielding CA, et al. 2008. J. Immunol. 181:2189.
5. Sonderegger I, et al. 2008. Eur. J. Immunol. 38:1833. - Gene ID
- 16193 View all products for this Gene ID
- UniProt
- View information about IL-6 on UniProt.org