- Regulatory Status
- RUO
- Other Names
- Interleukin-6, Interferon-β2, B cell stimulating factor-2 (BSF-2), Cytotoxic T cell differentiation factor (CDF), Hepatocyte stimulating factor (HSF), Hybridoma/plasmacytoma growth factor (HPGF)
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Recombinant Mouse IL-6 induces the proliferation of mouse 7TD1 cells in a dose dependent manner. The ED50 for this effect is < 10 pg/mL. -
Stability Testing for Recombinant Mouse IL-6. Recombinant mouse IL-6 was aliquoted in 0.1% TFA (trifluoroacetic acid), 10% ACN (Acetonitrile) at 0.2 mg/mL and one aliquot was kept at 4°C (Control), and another was frozen and thawed four times (4x Freeze/Thaw). The samples were tested by their property to induce the proliferation of mouse 7TD1 cells in a dose-dependent manner. The ED50 for this effect is < 10 pg/mL.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 575702 | 10 µg | $147.00 | |||
| 575704 | 25 µg | $265.00 | |||
| 575706 | 100 µg | $645.00 | |||
| 575708 | 500 µg | $1875.00 | |||
IL-6 is a multifunctional cytokine that can regulate various immune and inflammatory responses. Several studies have suggested a crucial role for IL-6 in angiogenesis. The use of mice deficient in IL-6 (-/-) demonstrated a critical role for this protein in a mouse model of lung angiogenesis. IL-6 has been shown to cause proliferation and migration of systemic endothelial cells in culture (1). The classical responsiveness to IL-6 is governed by a receptor complex consisting of two membrane-bound subunits, an 80-kD cognate chain (IL-6R), and a ubiquitously expressed 130-kD β-chain receptor (gp130) which acts as the universal signal-transducing element for all IL-6 family cytokines (2). Alternatively, IL-6 regulation of leukocyte trafficking relies upon signaling via its soluble IL-6R (termed IL-6 trans-signaling) (3). IL-6 plays a major role in regulating neutrophil clearance during acute peritoneal inflammation; as a result of specific down-regulation of neutrophil-attracting chemokine (CXCL1/KC) production (4). IL-6 is a key factor that reciprocally regulates Th17 and Foxp3(+) Treg differentiation by inhibition of TGF-beta induced Foxp3 and induction of RORgammat, a Th17 lineage-specific transcription factor (5).
Product Details
- Source
- Mouse IL-6, amino acids Phe25-Thr211 (Accession# NM_031168), was expressed in E. coli.
- Molecular Mass
- The 188 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 21,866 Da. The DTT-reduced protein migrates at approximately 22 kD and the non-reduced protein migrates at approximately 21.5 kD by SDS-PAGE.
- Purity
- Purity is >98%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in 0.1% TFA, 10% Acetonitrile
- Endotoxin Level
- Endotoxin level is <0.1 EU/µg (<0.01ng/µg) protein as determined by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Recombinant Mouse IL-6 induces the proliferation of mouse 7TD1 cells in a dose dependent manner. The ED50 for this effect is < 10 pg/mL.
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
- Additional Product Notes
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Get a 50% discount on this product when purchased in our Activation Bundles. Restrictions apply. Learn more…
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Application References
(PubMed link indicates BioLegend citation) - Product Citations
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Antigen Details
- Distribution
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IL-6 is released by activated T cells, B cells, monocytes, macrophages, fibroblasts, epithelial cells, and endothelial cells.
- Function
- Upregulated by IL-1, PDGF, IFN-β, TNF-α, NGF, IL-17; downregulated by glucocorticoids IL-4, TGF-β
- Interaction
- T cells, B cells, hepatocytes, cholinergic neurons
- Bioactivity
- Hematopoiesis, antigen-specific immune responses, inflammatory reactions, acute phase reactions
- Receptors
- Heterodimer IL-6Rα (CD126)/IL-6Rβ (CD130; gp130) and soluble IL-6R
- Cell Type
- Hematopoietic stem and progenitors
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Stem Cells
- Molecular Family
- Cytokines/Chemokines
- Antigen References
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1. McClintock JE and Wagner EM. 2005. 99:861-866
2. Murakami M. 1993. Science 260:1808-1810.
3. Jones SA,et al. 2001.J. FASEB. 15:43-58.
4. Fielding CA,et al. 2008. J. Immunol. 181:2189-2195.
5. Sonderegger I,et al. 2008. Eur. J. Immunol. 38:1833-1838. - Gene ID
- 16193 View all products for this Gene ID
- UniProt
- View information about IL-6 on UniProt.org