- Regulatory Status
- RUO
- Other Names
- SLAM family member 9, CD2 family member 10, CD2F-10, CD84 homolog 1, CD84-H1, SF2001.
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Mouse SLAMF9 induces the proliferation of mouse CD3-activated T cells in a dose-dependent manner. The ED50 for this effect is 0.15 - 0.9 µg/mL. -
Stability Testing for Recombinant Mouse SLAMF9. Recombinant mouse SLAMF9 was aliquoted in PBS, pH7 at 0.2 mg/mL. One aliquot was frozen and thawed four times (4x Freeze/Thaw) and compared to the control that was kept at 4°C (Control). The samples were tested for their ability to induce the proliferation of mouse CD3-activated T cells in a dose-dependent manner. The ED50 for this effect is 0.15 - 0.9 µg/mL.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 785602 | 10 µg | $112.00 | |||
| 785604 | 25 µg | $229.00 | |||
| 785606 | 100 µg | $675.00 | |||
| 785608 | 500 µg | $1465.00 | |||
SLAMF9 belongs to a member of the signaling lymphocytic activation molecule (SLAM) family and its structure shares homology with CD2 family members, including SLAMF5 (CD84), SLAMF3 (CD229) and SLAMF2 (CD48). It consists of two Ig-like domains in the extracellular region, a transmembrane domain and a cytoplasmic tail lacking SAP-binding motifs. SLAMF9 is expressed in macrophages, dendritic cells, B cells and T cells. SLAMF9-expressing tumor-associated macrophages (TAMs) were identified in human and mouse melanomas, and SLAMF9 expression appears to enhance TNF-α secretion upon LPS treatment and influences cell migration. Mice with double deficiency of SLAMF8 and SLAMF9 are highly resistant to LPS-induced liver injury by downregulation of TLR4 and reduction of secretion of inflammatory cytokines.
Product Details
- Source
- Mouse SLAMF9, amino acid (Phe18-Leu230) (Accession# Q9D780.2), with a C-terminal 6His tag, was expressed in 293E cells.
- Molecular Mass
- The 219 amino acid recombinant protein has a predicted molecular mass of approximately 24.47 kD. The DTT-reduced and non-reduced protein migrates at approximately 31-50 kD by SDS-PAGE. The predicted N-terminal amino acid is Phe.
- Purity
- >95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 μm filtered protein solution is in PBS pH7.2.
- Endotoxin Level
- Less than 0.1 EU per μg protein as determined by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- ED50 = 0.15 - 0.9 µg/mL, as determined dose dependent of anti-CD3-induced mouse T cell proliferation.
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Ig-containing molecules
- Distribution
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Predominantly restricted to hematopoietic tissue; monocytes, dendritic cells, B cells and T cells; cancer cells, tumor activated macrophages.
- Function
- SLAMF9 plays a role in the immune response. Induces pro-inflammatory cytokine secretion and migration in tumor-associated macrophages of murine and human melanoma.
- Interaction
- ND
- Bioactivity
- Mouse SLAMF9 induces the proliferation of mouse CD3-activated T cells in a dose-dependent manner.
- Cell Type
- B cells, Dendritic cells, Lymphocytes, Macrophages, T cells
- Biology Area
- Cancer Biomarkers, Cell Adhesion
- Antigen References
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- Fennelly JA, et al. 2001. Immunogenetics 53: 599.
- Fraser CC, et al. 2002. Immunogenetics 53: 843.
- Zhang W, et al. 2001. Clin Cancer Res 7: 822s.
- Dollt C, et al. 2018. Cell Death Dis 9: 939.
- Dragovich MA, et al. 2018. Autoimmun Rev 17: 674.
- Zeng X, et al. 2018. Cell Mol Immunol.
- Gene ID
- 98365 View all products for this Gene ID
- UniProt
- View information about SLAMF9 on UniProt.org