- Regulatory Status
- RUO
- Other Names
- TNFRSF13B
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Recombinant mouse TACI inhibits the proliferation of activated mouse B cells induced by recombinant mouse BAFF (Cat. No. 591202) in a dose-dependent manner. The ED50 for this effect is 10 – 100 ng/mL.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 577706 | 100 µg | $733.00 | |||
| 577708 | 500 µg | $1875.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
Transmembrane activator and calcium-modulator and cytophilin ligand interactor (TACI) is a TNFR family member molecule and is part of the so-called BAFF system. This includes two ligands: B cell-activating factor of the TNF family (BAFF) and a proliferation-inducing ligand (APRIL), and three receptors: BAFFR (TNFRSF13C), TACI (TNFRSF13B), and B cell maturation Ag (BCMA or TNFRSF17). BAFF binds the three receptors, while APRIL binds TACI and BCMA. TACI is constitutively expressed on naïve B cells and is upregulated on activated B cells. TACI levels are severely reduced in newborn mice B cells compared with those of adult mice, and newborn B cells do not secret Igs when they are stimulated with BAFF or APRIL. This reduced TACI expression might be responsible for the irresponsiveness of newborn B cells to T cell independent type II antigens such as bacterial capsular polysaccharides.
Product Details
- Source
- Mouse TACI (Accession# NM_021349: Met1-Thr 129) - SRENLYFQG - Human IgG (Pro 100 - Lys300) was expressed 293E cells.
- Molecular Mass
- The total predicted molecular weight is 41 kD. The non-reduced protein migrates between 80-90 kD and the DTT-reduced at approximately 45 - 50 kD by SDS-PAGE.
- Purity
- >98%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS
- Endotoxin Level
- Less than 0.01 ng per µg cytokine as determined by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Recombinant mouse TACI inhibits the proliferation of activated mouse B cells induced by recombinant mouse BAFF (Cat. No. 591202) in a dose-dependent manner. The ED50 for this effect is 10 – 100 ng/mL.
- Application
-
Bioassay
- Application Notes
-
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
- Product Citations
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Antigen Details
- Structure
- Disulfide linked homodimer
- Distribution
-
Peripheral B lymphocytes
- Function
- TACI triggers immunoglobulin class switching, IgG and IgA, by activating B cells through the adaptor MyD88. It also enhances the generation and maintenance of isotype-switched memory B lymphocytes and plasma cells, and increases the affinity of the produced antibody by the process of somatic hypermutation of the Ig gene. TACI drives plasma cell differentiation in LPS activated B cells. In addition, TACI is required in T-independent antibody production and in the regulation of B cell numbers. The interaction between B cells (expressing TACI) and DC (expressing BAFF) induces a rapid DC maturation which primes naive CD8+ T cells.
- Interaction
- B cells, myeloid cells, and monocytes derived dendritic cells.
- Ligand/Receptor
- APRIL (TNFSFL13A, CD256) and BAFF (TNFSFL13B, CD257) are TACI ligands.
- Cell Type
- B cells
- Biology Area
- Cell Biology, Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
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1. Schneider P, et al. 1999. J. Exp. Med. 189:1747.
2. Diaz-de-Durana Y, et al. 2006. Blood 107:594.
3. Kanswal S, et al. 2008. J. Immunol. 181:976.
4. Yang J, et al. 2009. PLOS One 4:e8494
5. Ozcan E, et al. 2009. J. Allergy Clin. Immunol. 123:1277.
6. He B, et al. 2010. Nat. Immunol. 1:836. - Gene ID
- 57916 View all products for this Gene ID
- UniProt
- View information about TACI on UniProt.org