- Regulatory Status
- RUO
- Other Names
- CED, DPD1, TGFB, TGFbeta, TGFB1
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Mouse TGF-β1 inhibits the proliferation of HT-2 cells induced by mouse IL-4.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 763102 | 10 µg | $223.00 | |||
| 763104 | 25 µg | $347.00 | |||
TGF-β1 is synthesized in cells as a 390-amino acid protein. Furin cleaves the protein at residue 278, yielding an N-terminal cleavage product which corresponds to the latency-associated peptide (LAP), and the 25 kD C-terminal portion of the precursor constitutes the mature TGF-β1. In addition to furin, some other activators can release TGF-β from LAP. TGF-β activators include proteases that degrade LAP, thrombospondin-1, reactive oxygen species, and the integrins αvβ6 and αvβ8. TGF-β is involved in regulating homeostasis of different Treg populations (naïve, inducible, peripheral) in both mouse and humans, although its specific roles remain the subject of active investigation. Th17 murine can be induced from naïve CD4+ T cells by the combination of TGF-β1 and IL-6 or IL-21. Nevertheless, the regulation of human Th17 differentiation is distinct. TGF-β1 seems to have dual effects on human Th17 differentiation in a dose-dependent manner. While TGF-β1 is required for the expression of RORct, in human naive CD4+ T cells from cord blood, TGF-β1 can inhibit the function of RORct at high doses. By using serum-free medium, it has been clarified that the optimum conditions for human Th17 differentiation are TGF-β1, IL-1β, and IL-2 in combination with IL-6, IL-21 or IL-23.
Product Details
- Source
- Mouse TGF-β1, amino acids (Ala279-Ser390) (Accession # NP_035707) was expressed in CHO cells.
- Molecular Mass
- The 112 amino acid recombinant protein has a predicted molecular mass of approximately 12.8kD. The DTT-reduced and non-reduced protein migrate at approximately 12.5 kD and 25 kD respectively by SDS-PAGE. The predicted N-terminal amino acid is Ala.
- Purity
- > 95% by SDS-PAGE gel as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in 30% Acetonitrile, 0.1% TFA (trifluoroacetic acid).
- Endotoxin Level
- Less than 0.01 ng/ per µg cytokine (0.1 EU/ µg) as determined by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Recommended Usage
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
- Product Citations
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Antigen Details
- Structure
- Dimer.
- Distribution
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TGF-β1 is secreted by numerous cells such as mesenchymal cells.
- Function
- Regulation of cell growth and survival, cell and tissue differentiation, development, inflammation, immunity, hematopoiesis, and tissue remodeling and repair. Essential for wound healing, stimulates matrix molecule deposition and angiogenesis, and essential mediator of the pathologic scarring in fibrotic disorders.
- Interaction
- Endothelial cells, mesenchymal cells, T cell, monocytes, and macrophages.
- Ligand/Receptor
- TGF-β1 binds to type II and type I serine/threonine kinase receptors, which initiate intracellular signals through activation of Smad proteins.
- Bioactivity
- Mouse TGF-β1 inhibits the proliferation of HT-2 cells induced by mouse IL-4. The ED50 = 0.05 — 0.25 ng/ml.
- Cell Type
- Embryonic Stem Cells, Mesenchymal Stem Cells, Neural Stem Cells
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Stem Cells
- Molecular Family
- Cytokines/Chemokines, Growth Factors
- Gene ID
- 21803 View all products for this Gene ID
- UniProt
- View information about TGF-beta1 on UniProt.org