- Regulatory Status
- RUO
- Other Names
- CD137, 4-1BB
-
Immobilized mouse TNFRSF9 binds TNFSF9 with an ED50 = 0.2 - 1.2 µg/ml. -
Immobilized mouse TNFRSF9 binds TNFSF9 in a dose dependent manner. BioLegend’s protein was compared side-by-side to the leading competitor’s equivalent product.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 756702 | 10 µg | $147.00 | |||
| 756704 | 25 µg | $347.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
TNFRSF9, also known as 4-1BB and CD137, is a member of the tumor necrosis factor receptor superfamily (TNFRSF). It is a costimulatory receptor similar to OX40, CD27, CD30, and CD40. The interaction of TNFRSF9 and its ligand, TNFSF9, leads to the activation of cytotoxic T cells, reactivation of anergic T lymphocytes, and induces T cell proliferation. TNFRSF9/TNFSF9 preferentially enhances the expansion of CD8+ T cells rather than CD4+ T cells, through the increase of IL-2Rα (CD25) and IL-2 expressions in CD8+ T cells. Among the TNFRSF members, TNFRSF9 is the most potent inducers of IL-2Rα expression on CD8+ T cells. In vivo studies with TNFSF9 knockout mice and with the agonistic anti-TNFRSF9 monoclonal antibody showed that TNFRSF9/TNFSF9 interaction keeps optimal CD8 T-cell mediated immune response against viral infections and tumors. The mouse TNFRSF9 has 60% identity to the human receptor at the amino acid level.
Product Details
- Source
- Mouse TNFRSF9, amino acids (Val24-Leu187) (Accession# NM_001077509), was expressed in 293E cells with a C-terminal human IgG-Fc-tag.
- Molecular Mass
- The 398 amino acid recombinant protein has a predicted molecular mass of approximately 43.9 kD. The protein migrates in DTT-reducing and non-reducing conditions at approximately 60 kD and 120 kD by SDS-PAGE respectively. The predicted N-terminal amino acid is Val.
- Purity
- >95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS, pH 7.2
- Endotoxin Level
- Less than 0.1 EU per µg of protein as determined by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Mouse TNFRSF9 activity was measured by a binding assay. Immobilized mouse TNFRSF9 binds TNFSF9 with an ED50 = 0.2 - 1.2 µg/ml.
- Application
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Bioassay
- Application Notes
-
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Dimer.
- Distribution
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Activated T cells, natural killer cells, macrophages, neutrophils, dendritic cells, eosinophils, Tregs, activated B cells, mast cells, and endothelial cells in tumor capillaries.
- Function
- Proliferation of T cells, activates their effector functions, survival, and establishes immunological memory.
- Interaction
- Activated antigen presenting cells, macrophages, B cells, B cell lymphoma, dendritic cells, a small fraction of T cells, epithelial, and endothelial cells.
- Ligand/Receptor
- TNFSF9 and CD137L.
- Bioactivity
- Mouse TNFRSF9 binds to TNFSF9.
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors, Soluble Receptors
- Antigen References
-
1. Pollok KE, et al. 1994. Eur. J. Immunol. 24:367.
2. Watts TH. 2005. Annu. Rev. Immunol. 23:23-68.
3. Zhu Y, et al. 2007. Blood 109:4882.
4. Locatelli F. 2009. Blood 11:2208.
5. Wang J, et al. 2010. J. Immunol. 185:7654.
6. Eun SY, et al. 2015. J. Immunol. 1:134-41.
7. Spencer AJ, et al. 2014. PLoS One 9:e105520.
8. Yi L, et al. 2014. PLoS One 1:e86337. - Gene ID
- 21942 View all products for this Gene ID
- UniProt
- View information about CD137 on UniProt.org