- Regulatory Status
- RUO
- Other Names
- VEGFA, Vegf, Vegf-a, Vegf120, Vpf
-
VegfA induces the proliferation of HUVEC cells
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 580902 | 10 µg | $229.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
VegfA belong to the VEGF family which has at least 7 members, VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, PlGF and snake venom-derived VEGFs such as T.f. (Trimeresurus flavoviridis) svVEGF. In humans, VEGF-A is highly expressed in most of the solid tumors generated in breast, lung, renal, colorectal and liver tissues. VEGF-A has strong vascular permeability activity, and significantly contributes to the formation of ascites tumors. VEGF can act as a direct proinflammatory mediator during the pathogenesis of RA, and protect rheumatoid synoviocytes from apoptosis, which contributes to synovial hyperplasia. VEGF is expressed in synovial macrophages and synovial fibroblasts in the synovial tissues of RA patients.
Product Details
- Source
- Mouse VEGF120, amino acids Ala27-Arg146 (Accession # S38100) was expressed in E. coli.
- Molecular Mass
- The 120 amino acid recombinant protein has a predicted molecular mass of approximately 14,071 Da. This protein exists as a disulfide-linked homodimer. The DTT-reduced protein migrates at approximately 15kDa and the non-reduced protein migrates as a homodimer, at approximately 30kDa by SDS-PAGE. The N-terminal amino acid is Alanine.
- Purity
- Purity is >98%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22µm filtered protein solution is in 5mM Citric Acid, 5mM NaH2PO4, 150mM NaCl, pH 4.0.
- Endotoxin Level
- Endotoxin level is <0.1 EU/µg (<0.01 ng/µg) protein as determined by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- ED50 = 1-4 ng/ml, corresponding to a specific activity of 1-0.25 x 106 units/mg, as determined by the dose dependent stimulation of HUVEC cells proliferation.
- Application
-
Bioassay
- Application Notes
-
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
- Product Citations
-
Antigen Details
- Structure
- cytokine
- Distribution
-
Widely expressed
- Function
- VegfA is a key player in vasculogenesis, the formation of blood vessels from progenitor cells, as well as angiogenesis. The expression of the Vegf-a gene is upregulated via hypoxia, estrogen, and NF-κB pathways. VegfA induces proliferation and cell migration in endothelial cells, and plays important roles during wound healing. In addition, VEGF regulates haematopoietic stem cell survival.
- Interaction
- VegfA interacts with vascular endothelial and monocytes/macrophages, which express VEGFR1. This interaction induces proliferation of endothelial cells, and stimulates migration of monocytes/macrophages. VEGFR2 is expressed in endothelial cells and VEGFR2-
- Ligand/Receptor
- Vascular endothelial growth factor (VEGF)-A binds and activates two tyrosine kinase receptors, VEGFR1 (Flt-1) and VEGFR2 (KDR/Flk-1).
- Cell Type
- Embryonic Stem Cells, Mesenchymal Stem Cells, Neural Stem Cells
- Biology Area
- Angiogenesis, Cell Biology, Neuroscience, Stem Cells, Synaptic Biology
- Molecular Family
- Cytokines/Chemokines, Growth Factors
- Antigen References
-
1. Shibuya M, et al. J Biochem Mol Biol 39:469-478 2006.
2. Shibuya M, et al. BMB Rep. 2008 41(4):278-86 2008.
3. Yoo SA, et al. Hindawi Publishing Corporation Mediators of Inflammation Volume 2008 Article ID 129873
4. Conn G, et al. Proc. Nati. Acad. Sci. 87:1323-1327 1990.
5. Gerber H, et al. Nature 417:954-958 2002. - Gene ID
- 22339 View all products for this Gene ID
- UniProt
- View information about VEGF on UniProt.org