- Regulatory Status
- RUO
- Other Names
- Colony stimulating factor 2, CSF2, Granulocyte/macrophage colony-stimulating factor, CSF-α, Pluripoietin-α, Eosinophil colony stimulating factor (Eo-CSF), Burst promoting activity (BPA)
-
Porcine GM-CSF induces the proliferation of human TF-1 cells in a dose-dependent manner. The ED50 for this effect is 1-6 ng/mL.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 782201 | 5 µg | $112.00 | |||
| 782203 | 20 µg | $253.00 | |||
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GM-CSF plays a key role in signaling emergency hemopoiesis (predominantly myelopoiesis) in response to infection, including the production of granulocytes and macrophages in the bone marrow and their maintenance, survival, and functional activation at sites of injury or insult. The receptor for GM-CSF is a heterodimer that comprises a major binding subunit (GMRα) and a major signaling subunit (βc). The receptor subunits are always coexpressed on the surface of leukocytes, with βc being expressed at lower levels than GMRα. Certain nonhemopoietic cell types have also been reported to express the GM-CSF receptor and to respond to GM-CSF stimulation in vitro, although the in vivo significance of these observations remains uncertain.
Product Details
- Source
- Porcine GM-CSF, amino acid Ala18-Lys144 (Accession # Q29118) was expressed in E.coli. The N-terminal contains Met.
- Molecular Mass
- The 128 amino acid recombinant protein has a predicted molecular mass of approximately 14.5 kD. The predicted N-terminal amino acid is Met.
- Purity
- >95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- Lyophylize from sterile 0.22 µm filtered protein solution is in PBS pH7.2.
- Endotoxin Level
- Less than 1 EU per μg protein as determined by the LAL method.
- Concentration
- Lyophilized recombinant protein is at 5 and 20 µg total protein
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. Reconstitute the protein in sterile water at 0.1 mg/mL. The protein can be aliquoted and stored at -20°C to -70°C. Stock solutions can also be prepared at 50 - 100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2 - 1% BSA or HSA and stored in working aliquots at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
- Activity
- Porcine GM-CSF induces the proliferation of human TF-1 cells in a dose-dependent manner. The ED50 for this effect is 1-6 ng/mL.
- Application
-
Bioassay
- Application Notes
-
BioLegend carrier-free recombinant proteins provided in liophylized format are shipped on blue ice.
Antigen Details
- Structure
- Cytokine
- Distribution
-
T cells, macrophages, fibroblasts, endothelial cells, mast cells
- Function
- Granulocyte-macrophage colony-stimulating factor (GM-CSF) and the related cytokines IL-3 and IL-5 regulate the production and functional activation of hemopoietic cells, with GM-CSF acting on monocyte/macrophages and all granulocytes.
- Interaction
- Granulocyte, macrophage, erythroid/megakaryocytic progenitors, myeloblasts, monoblasts, dendritic cells, T cells.
- Ligand/Receptor
- Heterodimer GM-CSFR α subunit (CDw116); β-subunit (CDw131) that is shared by IL-3 and IL-5 cytokines.
- Cell Type
- Embryonic Stem Cells, Granulocytes, Hematopoietic stem and progenitors
- Biology Area
- Cell Biology, Stem Cells
- Molecular Family
- Cytokines/Chemokines, Growth Factors
- Antigen References
-
- Hercus TR, et al. 2009 Blood 114:1289-98.
- Hayashida K, et al. 1990 Proc Natl Acad Sci USA. 87:9655-9.
- Walker F, et al. 1985 EMBO J. 4:933-9.
- Kitamura T, et al. 1989 J. Cell Physiol. 140:323.
- Hercus TR, et al. 2012 Growth Factors. 30:63.
- Gene ID
- 397208 View all products for this Gene ID
- UniProt
- View information about GM-CSF on UniProt.org