- Regulatory Status
- RUO
- Other Names
- Colony stimulating factor 2, CSF2, Granulocyte/macrophagecolony-stimulating factor, CSF-α, Pluripoietin-α, Eosinophil colonystimulating factor (Eo-CSF), Burst promoting activity (BPA)
-
Recombinant Porcine GM-CSF induces the proliferation of TF-1 cells in a dose-dependent manner. The ED50 for this effect is of 1 - 10 ng/mL. -
Stability Testing for Recombinant Porcine GM-CSF. Recombinant Porcine GM-CSF was aliquoted in PBS, pH 7.2 at 0.2 mg/mL and one aliquot was kept at 4°C (Control), and another was frozen and thawed four times (4x Freeze/Thaw). After this procedure, the samples were tested for their ability to induce the proliferation of TF-1 cells in a dose-dependent manner. The ED50 for this effect is 1 - 10 ng/mL.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 782204 | 25 µg | $725.00 | |||
| 782206 | 100 µg | $1270.00 | |||
GM-CSF plays a key role in signaling emergency hemopoiesis (predominantly myelopoiesis) in response to infection, including the production of granulocytes and macrophages in the bone marrow and their maintenance, survival, and functional activation at sites of injury or insult. The receptor for GM-CSF is a heterodimer that comprises a major binding subunit (GMRα) and a major signaling subunit (βc). The receptor subunits are always coexpressed on the surface of leukocytes, with βc being expressed at lower levels than GMRα. Certain nonhemopoietic cell types have also been reported to express the GM-CSF receptor and to respond to GM-CSF stimulation in vitro, although the in vivo significance of these observations remains uncertain.
Product Details
- Source
- Porcine GM-CSF, amino acid Ala18-Lys144 (Accession # Q29118.1) was expressed in E.coli
- Molecular Mass
- The 127 amino acid recombinant protein has a predicted molecular mass of approximately 14.4 kD. The DTT-reduced and non-reduced protein shall migrate at approximately 10-27 kD by SDS-PAGE. The predicted N-terminal amino acid is Met.
- Purity
- > 95%, as determined by Coomassie-stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS, pH 7.2.
- Concentration
- 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin the vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Porcine GM-CSF induces the proliferation of human TF-1 cells in a dose-dependent manner. The ED50 for this effect is 1 - 10 ng/mL.
- Application
-
Bioassay
- Application Notes
-
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Cytokine
- Distribution
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T cells, macrophages, fibroblasts, endothelial cells, mast cells
- Function
- Granulocyte-macrophage colony-stimulating factor (GM-CSF) and the related cytokines IL-3 and IL-5 regulate the production and functional activation of hemopoietic cells, with GM-CSF acting on monocyte/macrophages and all granulocytes.
- Interaction
- Granulocyte, macrophage, erythroid/megakaryocytic progenitors, myeloblasts, monoblasts, dendritic cells, T cells
- Ligand/Receptor
- Heterodimer GM-CSFR α subunit (CDw116); β-subunit (CDw131) that is shared by IL-3 and IL-5 cytokines
- Bioactivity
- Porcine GM-CSF induces the proliferation of human TF-1 cells in a dose-dependent manner. The ED50 for this effect is 1-10 ng/mL.
- Cell Type
- Embryonic Stem Cells, Granulocytes, Hematopoietic stem and progenitors
- Biology Area
- Cell Biology, Stem Cells
- Molecular Family
- Cytokines/Chemokines, Growth Factors
- Antigen References
-
- Hercus TR, et al. 2009. Blood. 114:1289-98.
- Hayashida K, et al. 1990. Proc Natl Acad Sci USA. 87:9655-9.
- Walker F, et al. 1985. EMBO J. 4:933-9.
- Kitamura T, et al. 1989. J Cell Physiol. 140:323-34.
- Hercus TR, et al. 2012. Growth Factors. 30:63-75.
- Gene ID
- 397208 View all products for this Gene ID
- UniProt
- View information about GM-CSF on UniProt.org