- Regulatory Status
- RUO
- Other Names
- B cell growth factor 1 (BCGF-1), B-cell stimulatory factor 1 (BSF-1), B cell growth factor 1, interleukin-4, lymphocyte stimulatory factor1
-
Immobilized porcine IL-4 Chimera induces the proliferation of TF-1 cells in a dose-dependent manner. The ED50 for this effect is of 0.04 – 0.4 ng/mL. -
Stability Testing for Recombinant Porcine IL-4 Chimera. Recombinant porcine IL-4 Chimera was aliquoted in PBS at 0.2 mg/mL and one aliquot was kept at 4°C (Control), and another was frozen and thawed four times (4x Freeze/Thaw). After this procedure, the samples were tested for their ability to induce the proliferation of TF-1 cells in a dose-dependent manner. The ED50 for this effect is 0.04 – 0.4 ng/mL.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 782824 | 25 µg | $405.00 | |||
| 782826 | 100 µg | $785.00 | |||
IL-4 is the primary cytokine implicated in the development of Th2-mediated responses, which is associated with allergy and asthma. The Type I receptor comprises IL-4Rα and the common gamma-chain (γc), which is also shared by the cytokines IL-2, -7, -9, -15, and -21 and is present in hematopoietic cells. IL-4 can use the type II complex, comprising IL-4Rα and IL-13Rα1, which is present in non-hematopoietic cells. This second receptor complex is a functional receptor for IL-13, which shares approximately 25% homology with IL-4. The type I receptor complex can be formed only by IL-4 and is active in Th2 development. In contrast, the type II receptor complex formed by either IL-4 or IL-13 is more active during airway hypersensitivity and mucus secretion and is not found in T cells.
Product Details
- Source
- Porcine IL-4, amino acid (His25-Cys133) (Accession #Q04745.1) with N-terminal Met, was expressed in E.coli.
- Molecular Mass
- The 110 amino acid recombinant protein has a predicted molecular mass of approximately 12.6 kD. The DTT-reduced and non-reduced protein shall migrate at approximately 6.5 -15 kD by SDS-PAGE. The predicted N-terminal amino acid is Met.
- Purity
- > 95%, as determined by Coomassie-stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS.
- Concentration
- 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Porcine IL-4 induces the proliferation of human TF-1 cells in a dose-dependent manner. The ED50 for this effect is 0.04– 0.4 ng/mL.
- Application
-
Bioassay
- Application Notes
-
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Cytokines
- Distribution
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IL-4 is produced by Th2 cells, naive CD4+ T cells, NKT cells, and basophils.
- Function
- IL-4 has a crucial role in the differentiation of Th2 cells and the induction of Th2-associated cytokines. IL-4, through its activation of STAT6, upregulates GATA3 expression and also suppresses Th1 and Th17 cell responses, partly through the upregulation of growth factor independent 1(GFI1), a transcriptional repressor of IFNγ and IL-17 production. IL-4 induces macrophage activation and TSLP production. IL-4 recruits and activates IgE-producing B cells (IgE class switching) and enhances IgE-mediated responses by up-regulating IgE receptors on B lymphocytes, mast cells, and basophils. In addition, IL-4 also induces VCAM-1 on vascular endothelium and thus directs the migration of T lymphocytes, monocytes, basophils, and eosinophils to the inflammation site.
- Interaction
- T cells, B cells, macrophages, epithelial cells, smooth muscle cells, and bronchial fibroblasts
- Ligand/Receptor
- IL-4 signals through Type I receptor complex (IL-4Rα, γc) and Type II receptor complex (IL-4Rα, IL-13Rα1).
- Bioactivity
- IL-4 signals through Type I (IL-4Rα, γc) and Type II (IL-4Rα, IL-13Rα1) receptor complexes.
- Cell Type
- Embryonic Stem Cells, Hematopoietic stem and progenitors
- Biology Area
- Cell Biology, Immunology, Stem Cells
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
- Swain SL, et al. 1990. J Immunol. 145:3796-806.
- Hsieh CS, et al. 1992. P Natl Acad Sci USA. 89:6065.
- Allison-Lynn A, et al. 2006. J Immunol. 176:7456.
- Kato A, et al. 2007. J Immunol. 179:1080-7.
- LaPorte SL, et al 2008. Cell. 132:259-72.
- Martinez FO, et al. 2009. Annu Rev Immunol. 27:451-83.
- Gene ID
- 397225 View all products for this Gene ID
- UniProt
- View information about IL-4 on UniProt.org