- Regulatory Status
- RUO
- Other Names
- FGF-2, HBGF-2, BFGF, FGFB, Fibroblast Growth Factor 2, Basic Fibroblast Growth Factor, Prostatropin, Heparin-Binding Growth Factor 2
-
Recombinant Rat FGF-basic induces proliferation of NIH-3T3 cells in a dose-dependent manner. The ED50 for this effect is 0.4 – 4.0 ng/mL. -
Stability Testing for Recombinant Rat FGF-basic.
Recombinant Rat FGF-basic was aliquoted in 10 mM MOPS, 50 mM Na2SO4,, 0.5 mM EDTA, 0.5 mM DTT, pH 7.2 at 0.2 mg/mL and one aliquot was kept at 4°C (Control), and another was frozen and thawed four times (4x Freeze/Thaw). After this procedure, the samples were tested for their ability to induce cell proliferation in NIH- 3T3 cells in a dose-dependent manner. The ED50 for this effect is 0.4 – 4.0 ng/mL.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 567304 | 25 µg | $260.00 | |||
| 567306 | 100 µg | $410.00 | |||
FGF-basic also known as FGF-2, is a member of the fibroblast growth factor (FGF) family, which includes 23 members. FGF-2 is expressed in almost all tissues and plays an important role in a variety of normal and pathological processes, including development, wound healing, and neoplastic transformation. FGF-2 is mitogenic for many cell types, including epithelial and mesenchymal cells. It shows potent angiogenic activity and has been implicated in tumor angiogenesis. FGF-2 significantly promotes the proliferation of adipose-derived mesenchymal cells (AMC) and enhances chondrogenesis in three-dimensional micromass culture. FGF-2 binds to a family of four distinct, high affinity tyrosine kinase receptors, designated FGFR-1 to FGFR-4. In addition, FGF-2 binds to the extracellular matrix (ECM) and heparan sulfate (HS), and is an essential and dynamic regulator of fibroblast growth factor (FGF) signaling. At the molecular level, two fundamentally different crystallographic models have been proposed to explain how heparin sulfate enables FGF and FGF receptor (FGFR) to assemble into a functional dimer on the cell surface. FGF-2, αvβ3 integrin, and FGFR- 1, form a trimolecular complex required for ERK1/2 activation. MT1- MMP (MMP-14) downregulates the amount of FGF-2 bound to the cell surface and therefore, reduces FGF-2 signaling.
Product Details
- Source
- Rat FGF-basic (FGF-2), amino acids (Ala11-Ser154) (Accession # P13109.1), was expressed in E. coli.
- Molecular Mass
- The 144 amino acid recombinant protein has a predicted molecular mass of approximately 16.2 kD. The DTT-reduced and non-reduced protein migrates at approximately 17-25 kD by SDS-PAGE. The predicted N-terminal amino acid is Ala.
- Purity
- > 95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 μm filtered protein solution is in 10 mM MOPS, 50 mM Na2SO4, 0.5 mM EDTA, 0.5 mM DTT, pH 7.2
- Endotoxin Level
- Less than 0.1 EU per µg cytokine as determined by the LAL method.
- Concentration
- 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 μg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Recombinant Rat FGF-basic induces proliferation of NIH-3T3 cells in a dose-dependent manner. The ED50 for this effect is 0.4 - 4.0 ng/mL.
- Application
-
Bioassay
- Application Notes
-
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Monomer
- Distribution
-
Brain, retina, pituitary, kidney, placenta, testis, corpus luteum, adrenal glands, monocytes, prostate, bone, liver, cartilage, endothelial cells, and epithelial cells
- Function
- Possess broad mitogenic and potent angiogenic activity, plays a key role in physiological and pathological conditions, including embryonic development, wound repair, inflammation, and tumor growth. MT1-MMP downregulates fibroblast growth factor-2 (FGF-2) signaling.
- Interaction
- Fibroblasts, myoblasts, osteoblasts, neuronal cells, endothelial cells, keratinocytes, chondrocytes, astrocytes, oligodendrocytes, and smooth muscle cells
- Ligand/Receptor
- FGFR1, FGFR2, FGFR3 and FGFR4. Low affinity coreceptor heparan sulfate (HS) and heparan sulfate proteoglycans (HSPG) required for full activity.
- Bioactivity
- Recombinant Rat FGF-basic induces proliferation of NIH-3T3 cells in a dose-dependent manner. The ED50 for this effect is 0.4 - 4.0 ng/mL.
- Cell Type
- Endothelial cells, Epithelial cells, Monocytes
- Biology Area
- Angiogenesis, Cell Proliferation and Viability, Signal Transduction
- Molecular Family
- Growth Factors
- Antigen References
-
- Schlessinger J, et al. 2000. Mol Cell. 6:743-50.
- Ibrahimi OA, et al. 2001. Proc Natl Acad Sci U S A. 98:7182-7.
- Beenken A & Mohammadi M. 2009. Nat Rev Drug Discov. 8:235-53.
- Tassone E, et al. 2015. J Cell Physiol. 230:366-77.
- Chien SY, et al. 2016. Clin Sci (Lond). 130:667-81.
- Gene ID
- 54250 View all products for this Gene ID
- UniProt
- View information about FGF-basic on UniProt.org