- Regulatory Status
- RUO
- Other Names
- Granulocyte Colony Stimulating Factor, Colony Stimulating Factor 3 (CSF3), GCSF, Pluripoietin, Lenograstim
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Rat G-CSF induces the proliferation of M-NFS-60 mouse myelogenous leukemia cells. The proliferation was measured using Deep Blue Cell Viability™ Kit (Cat. No. 424701). The ED50 = 0.02 - 0.1 ng/ml. -
Stability testing for rat G-CSF. Rat G-CSF was aliquoted in PBS at 0.2 mg/ml and one aliquot was kept at 4°C (control) and another was freeze-thawed four times (4 x freeze-thaws). After this procedure, the samples were tested by their property to induce M-NFS-60 60 mouse myelogenous leukemia cell proliferation.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 775002 | 10 µg | $211.00 | |||
| 775004 | 25 µg | $352.00 | |||
G-CSF is the key hematopoietic cytokine involved in the control of neutrophil development; therefore it is a critical regulator of innate immunity against bacterial infections. G-CSF mobilizes stem cells indirectly by down-regulating the expression of CXCL12 on marrow osteoblasts and by releasing neutrophil and monocyte proteolytic enzymes, including neutrophil elastase, cathepsin G, and matrix metalloproteinase-9, which in turn degrade important HSC-trafficking and adhesion molecules CD117 (c-kit), CD106 (VCAM-1), and CD184 (CXCR4). In addition, G-CSF possesses immunosuppressive effects on monocytes/macrophages, dendritic cells, and T lymphocytes. The receptor for G-CSF is expressed not only in hematopoietic cells; but also in cardiomyocytes, skeletal muscle, and neurons. G-CSF influences mouse skeletal muscle development and regeneration by stimulating myoblast proliferation. In vitro, G-CSF displays strong antiapoptotic activity in neuronal cells. Also, G-CSF has pleiotropic actions in the central nervous system. It has been reported to have neuroprotective effect in focal cerebral ischemia models and it counteracts programmed cell death and drives neurogenesis.
Product Details
- Source
- Rat G-CSF amino acids (Ile22 – Ile214) (Accession # NM_017104.1) was expressed in CHO cells.
- Molecular Mass
- The 193 amino acid recombinant protein has a predicted molecular mass of approximately 21.2 kD. The DTT-reduced and non-reduced protein migrate at approximately 25 kD by SDS-PAGE. The predicted N-terminal amino acid is Ile.
- Purity
- > 95% by SDS-PAGE gel as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS pH 7.2.
- Endotoxin Level
- Less than 1.0 EU per µg cytokine as determined by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Rat G-CSF induces the proliferation of M-NFS-60 mouse myelogenous leukemia cells. The proliferation was measured using Deep Blue Cell Viability™ Kit (Cat. No. 424701). The ED50 = 0.02 - 0.1 ng/ml.
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
- Product Citations
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Antigen Details
- Structure
- Heterodimer
- Distribution
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Monocytes, mesothelial cells, fibroblasts, endothelial cells, and neurons.
- Function
- G-CSF acts on myeloid progenitor cells to stimulate survival, proliferation, and neutrophilic maturation; on mature neutrophils, G-CSF enhances survival, superoxido anion and alkaline phosphatase production, arachidonic acid release, and antibody dependent cellular cytotoxicity.
- Interaction
- Hematopoietic progenitor cells, neutrophilic granulocytes, monocytes, platelets, endothelial cells, trophoblastic cells, myoblasts, cardiomyocytes, neurons, and some small cell lung carcinoma cell lines.
- Ligand/Receptor
- G-CSFR.
- Bioactivity
- Rat G-CSF induces the proliferation of M-NFS-60 cell.
- Cell Type
- Hematopoietic stem and progenitors, Embryonic Stem Cells
- Biology Area
- Immunology, Innate Immunity, Stem Cells
- Molecular Family
- Growth Factors, Cytokines/Chemokines
- Antigen References
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- Schneider A, et al. 2005. J Clin Invest. 115:2083.
- Kim HK, et al. 2006. Blood 108:812.
- Donahue RE, et al. 2009. Blood 114:2530.
- Shimoji K, et al. 2010. Cell. Stem. Cell. 6:227.
- Martins A, et al. 2010. IUBMB Life 62:611.
- Hara M, et al. 2011. J Exp Med. 208:715.
- Kadota R, et al. 2012. PLoS One. 7:e50391.
- Gene ID
- 25610 View all products for this Gene ID
- UniProt
- View information about G-CSF on UniProt.org