- Regulatory Status
- RUO
- Other Names
- IFN-gamma, IFNG2
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Recombinant rat IFN-γ induces cytotoxicity in L929 cells in a dose dependent manner. The ED50 of this effect is 0.2 - 1.4 ng/mL
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 598802 | 10 µg | $54.00 | |||
| 598804 | 25 µg | $112.00 | |||
| 598806 | 100 µg | $223.00 | |||
| 598808 | 500 µg | $750.00 | |||
IFN-γ is a key component of the immune response to viral infections. It is produced by NK, NKT, and γ/δ T cells during early stages of infection and by CD4 and CD8 T cells later during the adaptive immune response. During infection, the role of IFN-γ includes protection, clearance, and modulation of the immune response. IFN-γ modulates epithelial cell functions and may play a role in intestinal wound healing as it might help preserve the integrity of the intestinal epithelium. It has been published that IFN-γ enhances IL-27 secretion, and the treatment of rats in a model of arthritis, IFN-γ/IL-27 successfully controlled arthritic inflammation and inhibited mediators of inflammation, angiogenesis, cell survival, apoptosis, and tissue damage. Nevertheless, IFN-γ has been associated with multiple inflammatory and autoimmune diseases such as arthritis, lupus nephritis, diabetic retinopathy, lung fibrosis, allergic conjunctivitis, and pediatric SLE. Rat IFN-γ protein has 39% homology with humans and 85% homology with mice proteins. Due to the high homology between rat and mouse IFN-γ, there is a strong affinity for rat IFN-γ on mouse cells.
Product Details
- Source
- Rat IFN-γ, amino acids Met-(Gln23-Cys156) (Accession# NM_138880) was expressed in E. coli.
- Molecular Mass
- The 135 amino acid recombinant protein has a predicted molecular mass of approximately 15.6 kD. The DTT-reduced and non-reduced protein migrate at approximately 14 kD by SDS-PAGE. The N-terminal amino acid is Met.
- Purity
- >95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in 20 mM HEPES, 150 mM NaCl, 5 mM TCEP, pH 7.2.
- Endotoxin Level
- Less than 0.01 ng per µg cytokine as determined by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Recombinant rat IFN-γ induces cytotoxicity in L929 cells in a dose dependent manner. The ED50 of this effect is 0.2 - 1.4 ng/mL
- Application
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Bioassay
- Application Notes
-
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
- Product Citations
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Antigen Details
- Distribution
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NK and NKT cells, gamma/delta T cells, CD4, CD8 T cells.
- Function
- IFN-γ has antiviral, antiproliferative, and immunomodulatory properties. IFN-γ, in conjunction with IL-12 and T-bet transcription factor, is essential in TH1 cell differentiation. IFN-γ is synergistically induced by IL-21 and IL-15 or IL-18 in NK T cells. Also, IFN-γ is synergistically induced by IL-12 and IL-18 in T and NK cells.
- Interaction
- T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts.
- Ligand/Receptor
- Homodimer form by IFNGR1 and IFNGR2.
- Bioactivity
- Antiviral activity on L929 cells using EMC virus.
- Cell Type
- Embryonic Stem Cells
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Stem Cells
- Molecular Family
- Cytokines/Chemokines
- Antigen References
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1. Dijkema R, et al. 1985. EMBO 4:761.
2. Wang T, et al. 2003. J. Immunol. 171:2524.
3. Strengell M, et al. 2003. J. Immunol. 170:5464.
4. Rajaiah R, et al. 2011. J. Biol. Chem. 286:2817.
5. Rana A, et al. 2012. Lupus 21:1105.
6. Lee EY, et al. 2013. PLoS One 8:e76412. - Gene ID
- 25712 View all products for this Gene ID
- UniProt
- View information about IFN-gamma on UniProt.org