- Regulatory Status
- RUO
- Other Names
- IL-1RA, IL-1F3, ICIL-1RA, IRAP, IL-1RN, DIRA
-
Recombinant rat IL-1RA (IL-1RN) inhibits the proliferation of D10.G4.1 cells induced by recombinant human IL-1α (Cat. No. 570002) at 50 pg/mL.. The EC50 range for this effect is 3 - 30 ng/mL. -
Stability testing for Recombinant Rat IL-1RA (IL-1RN). Recombinant Rat IL-1RA (IL-1RN) was aliquoted in PBS, pH 7.2, 5 mM DTT at 0.2 mg/mL and one aliquot was kept at 4°C (Control), and another was frozen and thawed four times (4x Freeze/Thaw). The samples were tested by their ability to inhibit D10.G4.1 cell proliferation induced by recombinant human IL-1α (Cat. No. 570002) at 50 pg/mL in a dose-dependent manner. The EC50 range for this effect is 3 - 30 ng/mL.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 560504 | 25 µg | $370.00 | |||
| 560506 | 100 µg | $605.00 | |||
Interleukin-1 receptor antagonist (IL-1RA) is a member of the IL-1 family that binds to IL-1 receptors. IL-1RA is a naturally-occurring, inflammatory-inhibitor protein. IL-1 receptor antagonist, IL-1RA/IL-1RN, was initially purified from media conditioned by human monocytes. IL-1RA belongs to the IL-1 family of cytokines, which includes 11 members, including the IL-1RA, sub-divided into three subfamilies. The IL-1 subfamily (IL-1α, IL-1β, IL-33), the IL-18 subfamily (IL-18 and IL-37), and the IL-36 subfamily (IL-36Rα, IL-36α, β, γ, and IL-38). Although all members are secreted proteins, IL-1RA is the only one that possesses a signal peptide, and it is secreted via the endoplasmic reticulum and Golgi apparatus. In addition to the glycosylated secreted IL-1RA, an intracellular un-glycosylated isoform has also been described. The two forms of IL-1RA are transcribed from the same gene through the use of alternative first exons. IL-1RA competitively binds to the IL-1 type 1 receptor (IL-1RI) to prevent its binding with two distinct ligands (IL-1α and IL-1β), which recruits an Interleukin-1 receptor accessory protein (IL-1RAP). IL-1RA exhibits potent anti-inflammatory properties in vivo and can mediate protective effects against inflammatory diseases. IL-1RA has been used in controlled clinical trials for the treatment of rheumatoid arthritis (RA). It has been reported that it reduces clinical signs and symptoms associated with the disease. IL-1RA (Kineret® , Anakinra) has also been used in the treatment of Type 2 Diabetes.
Product Details
- Source
- Recombinant rat IL-1Rα, amino acid His27-Gln178 (Accession No. P25086.1) was expressed in E.coli
- Molecular Mass
- The 152 amino acid recombinant protein has a predicted molecular mass of approximately 17.5 kD. The DTT-reduced and non-reduced protein migrates at approximately 15 - 20 kD by SDS-PAGE.
- Purity
- > 95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS, pH7.2, 5 mM DTT
- Endotoxin Level
- Less than 0.1 EU per µg cytokine as determined by the LAL method.
- Concentration
- 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Recombinant Rat IL-1RA inhibits the proliferation of D10.G4.1 cells induced by recombinant human IL-1α (Cat. No. 570002) at 50 pg/mL. The EC50 range for this effect is 3 - 30 ng/mL.
- Application
-
Bioassay
- Application Notes
-
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Monomor
- Distribution
-
Monocytes, neutrophils, macrophages, endothelial cells, corneal epithelial cells, oral mucosa epithelial cells, fibroblasts
- Function
- Inhibits the activity of IL-1β and IL-1α by binding to the receptor IL-1RI and blocking the downstream signaling. IL-1RA is induced by IL-1, LPS, IgG complexes, IL-4, and IL-10.
- Interaction
- T cells, fibroblasts, endothelial cells, epithelial cells, monocytes, dendritic cells, cardiomyocytes
- Ligand/Receptor
- Type I and II IL-1 receptors (IL-1RI and IL-1RII)
- Bioactivity
- Recombinant rat IL-1RA inhibits the proliferation of D10.G4.1 cells
- Cell Type
- Epithelial cells, Fibroblasts, Macrophages, Monocytes, Neutrophils
- Biology Area
- Immuno-Oncology, Immunology, Innate Immunity, Signal Transduction
- Molecular Family
- Cytokines/Chemokines, Cytoskeletal Proteins
- Antigen References
-
- Hannum CH, et al. 1990. Nature. 343:336-40.
- Cohen S, et al. 2002. Arthritis Rheum. 46:614-24.
- Arend WP. 2002. Cytokine Growth Factor Rev. 13:323-40.
- Botsios C, et al. 2007. Reumatismo. 59:32-7.
- Larsen CM, et al. 2007. N Engl J Med. 356:1517-26.
- Dinarello CA. 2018. Immunol Rev. 281:8-27.
- Butcher C, et al. 1994. J Immunol. 153:701-11.
- Smith MJ, et al. 1992. J Immunol. 149:2000-7.
- Gene ID
- 60582 View all products for this Gene ID
- UniProt
- View information about IL-1RA on UniProt.org