- Regulatory Status
- RUO
- Other Names
- T-cell grow factor, TCGF
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CTLL-2 proliferation induced by rat IL-2
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 579502 | 10 µg | $147.00 | |||
| 579504 | 25 µg | $265.00 | |||
| 579506 | 100 µg | $645.00 | |||
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Interleukin 2 was discovered through its function as a T cell growth factor (TCGF). IL-2 binds to IL-2 receptor which is expressed in T and B cells, thymocytes, and NK cells. The IL-2 receptor comprises three distinct components: the α-chain, which is cytokine specific, and the β- and γc-subunits which are share with the IL-15 receptor. In addition, the γc-subunit is a component of a series of other cytokine receptors, these being members of the γc cytokine receptor family (IL-4, IL-7, IL-9, and IL-21) (3). IL-2 signaling may play a major role in the differentiation of regulatory T cells (4). IL-2, IL-15, and IL-7 can all support NK cell differentiation; nevertheless, analyses of IL-2(-/-), IL-2Rα(-/-) mice fail to exhibit significant defects in NK cell development. This data suggest that IL-2 might have a redundant role in NK cell differentiation (4).
Product Details
- Source
- Rat IL-2, amino acids Ala21-Met153 (Accession # NM_053836) was expressed in E. coli.
- Molecular Mass
- The 134 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 15,396 Da. The DTT-reduced protein migrates at approximately 14kDa and the non-reduced protein migrates with slightly greater mobility by SDS-PAGE.
- Purity
- Purity is >98%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in 5mM NaH2PO4, 5mM citric acid, 150mM NaCl, pH 4.0.
- Endotoxin Level
- Endotoxin level is <0.1 EU/µg (<0.01ng/µg) protein as determined by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
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Assay 1. Recombinant Rat IL-2 induces the proliferation of mouse CTLL2 cells in a dose-dependent manner. The ED50 for this effect is 0.1 to 0.6 ng/mL.
Assay 2. Recombinant Rat IL-2 induces the proliferation of mouse HT-2 cells in a dose-dependent manner. The ED50 for this effect is 0.15 to 0.9 ng/mL. - Application
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Bioassay
- Recommended Usage
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Use when high specific biological activity is required.
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
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Application References
(PubMed link indicates BioLegend citation) -
- Xiao X,. et al. 2012. J. Immunol. 188:892. PubMed.
- Product Citations
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Antigen Details
- Structure
- cytokine
- Distribution
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Activated T cells are the predominant source of IL-2 produced during immune responses
- Function
- IL-2 stimulates the proliferation of T cells, induces the generation of cytotoxic T lymphocytes (CTLs), facilitates the proliferation of and the synthesis of immunoglobulin by B cells, and induces the generation and persistence of natural killer (NK) cells. In addition, IL-2 signaling plays a major role in the differentiation of regulatory T cells.
- Ligand/Receptor
- IL-2R (α, β, γc)
- Cell Type
- Hematopoietic stem and progenitors, Embryonic Stem Cells
- Biology Area
- Cell Biology, Immunology, Stem Cells
- Molecular Family
- Cytokines/Chemokines
- Antigen References
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1. Lowenthal JW et al, Nature 315:669-672 1985.
2. Waldman TA et al, Nat Rev Immunol 6:595-601 2006
3. de Bakker BI et al, J Cell Science 121:627-633 2008
4. Averil M et al, Annu. Rev. Immunol 24:657-679 2006. - Gene ID
- 116562 View all products for this Gene ID
- UniProt
- View information about IL-2 on UniProt.org