- Regulatory Status
- RUO
- Other Names
- B cell growth factor 1 (BCGF-1), B-cell stimulatory factor 1 (BSF-1), interleukin-4, lymphocyte stimulatory factor 1
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Recombinant rat IL-4 induces the proliferation of TF 1 human erythroleukemic cells in a dose dependent manner. The ED50 for this effect is 0.3 – 1.5 µg/mL. -
Stability testing for recombinant rat IL-4. Recombinant rat IL-4 was aliquoted in PBS, pH 7.2 at 0.2 mg/mL and one aliquot was kept at 4°C (control), and another was frozen and thawed four times (4x Freeze/thaws). After this procedure, the samples were tested for their ability to induce TF-1 cell proliferation. -
Recombinant rat IL-4 induces the proliferation of TF 1 human erythroleukemic cells in a dose dependent manner. BioLegend’s protein was compared side-by-side to a competitor’s equivalent product.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 776902 | 10 µg | $94.00 | |||
| 776904 | 25 µg | $229.00 | |||
| 776906 | 100 µg | $675.00 | |||
IL-4 is the primary cytokine implicated in the development of Th2-mediated responses, which is associated with allergy and asthma. The Type I receptor comprises IL-4Rα and the common gamma-chain (γc), which is also shared by the cytokines IL-2, -7, -9, -15 and -21 and is present in hematopoietic cells. IL-4 can use the type II complex, comprising IL-4Rα and IL-13Rα1, which is present in non-hematopoietic cells. This second receptor complex is a functional receptor for IL-13, which shares approximately 25% homology with IL-4. The type I receptor complex can be formed only by IL-4 and is active in Th2 development. In contrast, the type II receptor complex formed by either IL-4 or IL-13 is more active during airway hypersensitivity and mucus secretion and is not found in T cells.
Product Details
- Source
- Rat IL-4 amino acids (Ile22 – Ser147) (Accession # P20096) was expressed in 293E. The amino terminal contain 8His-3GSIEGR- tag.
- Molecular Mass
- The 142 amino acid recombinant protein has a predicted molecular mass of approximately 15.8 kD. The protein migrates approximately at 23 kD in DTT-reducing and non-reducing conditions by SDS-PAGE. The predicted N-terminal amino acid is His.
- Purity
- > 98% by SDS-PAGE gel and HPLC analyses.
- Formulation
- 0.22 µm filtered protein solution is in PBS pH 7.2.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Rat IL-4 induces the proliferation of TF-1 human erythroleukemic cells in a dose dependent manner. The ED50 is 0.3 - 1.5 µg/mL.
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
- Product Citations
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Antigen Details
- Structure
- Heterodimer
- Distribution
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IL-4 is produced by Th2 cells, naive CD4+ T cells, NKT cells, and basophils.
- Interaction
- T cells, B cells, macrophages, epithelial cells, smooth muscle cells, and bronchial fibroblasts.
- Ligand/Receptor
- IL-4 signals through Type I (IL-4Rα, γc) and Type II receptors (IL-4Rα, IL-13Rα1) complexes.
- Bioactivity
- IL-4 has a crucial role in the differentiation of TH2 cells and induction of Th2 associated cytokines. IL-4, through its activation of STAT6, upregulates GATA3 expression and also suppresses TH1 and TH17 cell responses, partly through the upregulation of
- Cell Type
- B cells, Hematopoietic stem and progenitors, Embryonic Stem Cells
- Biology Area
- Cell Biology, Immunology, Stem Cells
- Molecular Family
- Cytokines/Chemokines
- Antigen References
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- Richter G, et al. 1990. Cytokine 2:221.
- Hsieh CS, et al. 1992. Proc. Natl. Acad. Sci. USA. 89:6065.
- Andrews AL, et al. 2006. J. Immunol. 176:7456.
- Kato A, et al. 2007. J. Immunol. 179:1080.
- LaPorte SL, et al. 2008. Cell. 132:259.
- Martinez FO, et al. 2009. Annu. Rev. Immunol. 27:451.
- Gene ID
- 287287 View all products for this Gene ID
- UniProt
- View information about IL-4 on UniProt.org