- Regulatory Status
- RUO
- Other Names
- Interleukin-6, Interferon-β2, B cell stimulating factor-2 (BSF-2), Cytotoxic T cell differentiation factor (CDF), Hepatocyte stimulating factor (HSF), Hybridoma/plasmacytoma growth factor (HPGF)
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Recombinant rat IL-6 induces the proliferation of mouse 7TD1 cells in a dose dependent manner. The ED50 for this effect is 0.6 – 6 pg/mL. -
Stability Testing for Recombinant Rat IL-6. Recombinant rat IL-6 was aliquoted in 0.1% trifluoro acetic acid, 10% Acetonitrile and one aliquot was kept at 4°C (Control), and another was frozen and thawed four times (4x Freeze/Thaw). The samples were tested by their property induce the proliferation of of mouse 7TD1 cells in a dose-dependent manner. The ED50 for this effect is 0.6 – 6.0 pg/mL.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 760002 | 10 µg | $147.00 | |||
| 760004 | 25 µg | $265.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
IL-6 is a multifunctional cytokine that can regulate various immune and inflammatory responses. IL-6 was initially identified as a B-cell differentiation factor, capable of inducing the maturation of B cells into antibody-producing cells. IL-6 is the major circulating pyrogen during inflammation or injury. Several studies suggested a crucial role for IL-6 in angiogenesis. The use of mice deficient in IL-6 (–/–) demonstrated a critical role for this protein in a mouse model of lung angiogenesis. IL-6 has been shown to cause the proliferation and migration of systemic endothelial cells in culture. The classical responsiveness to IL-6 is governed by a receptor complex consisting of two membrane-bound subunits, an 80 kD cognate-chain (IL-6R), and a ubiquitously expressed 130 kD β-chain receptor (gp130), which acts as the universal signal-transducing element for all IL-6 family cytokines. Alternatively, IL-6 regulation of leukocyte trafficking relies upon the signaling via its soluble IL-6R (termed IL-6 trans-signaling). IL-6 plays a major role in regulating neutrophil clearance during acute peritoneal inflammation as a result of specific down-regulation of neutrophil-attracting chemokine (CXCL1/KC) production. IL-6 is a key factor that reciprocally regulates Th17 and FOXP3(+) Treg differentiation by the inhibition of TGF-β induced FOXP3 and induction of RORγt, which is a Th17 lineage-specific transcription factor.
Product Details
- Source
- Rat IL-6, amino acids Met-(Phe25-Thr211) (Accession# NM_012589), was expressed in E. coli.
- Molecular Mass
- The 188 amino acid recombinant protein has a predicted molecular mass of approximately 21.8 kD. The DTT-reduced and non-reduced protein migrates at approximately 22 kD by SDS-PAGE. The predicted N-terminal amino acid is Met.
- Purity
- >95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in 0.1% TFA, 10% Acetonitrile
- Endotoxin Level
- Less than 0.01 ng per µg cytokine as determined by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Recombinant rat IL-6 induces the proliferation of mouse 7TD1 cells in a dose dependent manner. The ED50 for this effect is 0.6 – 6 pg/mL.
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Growth factor.
- Distribution
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IL-6 is released by activated T cells, B cells, monocytes, macrophages, fibroblasts, epithelial cells, and endothelial cells.
- Function
- Upregulated by IL-1, PDGF, IFN-β, TNF-α, NGF, and IL-17 and is downregulated by glucocorticoids IL-4 and TGF-β.
- Interaction
- T cells, B cells, hepatocytes, and cholinergic neurons.
- Ligand/Receptor
- Heterodimer IL-6Rα (CD126)/IL-6Rβ (CD130; gp130) and soluble IL-6R.
- Cell Type
- Hematopoietic stem and progenitors
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Stem Cells
- Molecular Family
- Cytokines/Chemokines
- Antigen References
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1. McClintock JY, Wagner EM. 2005. J. Appl. Physiol. 99:861.
2. Murakami M, et al. 1993. Science 260:1808.
3. Cartmell T, et al. 2000. J. Physiol. 526:653.
4. Jones SA, et al. 2001. FASEB J. 15:43.
5. Fielding CA, et al. 2008. J. Immunol. 181:2189.
6. Sonderegger I, et al. 2008. Eur. J. Immunol. 38:1833.
7. Erta M, et al. 2012. Int. J. Biol. Sci. 8:1254.
8. Rosean TR, et al. 2014. Immunol. Res. 59:188. - Gene ID
- 24498 View all products for this Gene ID
- UniProt
- View information about IL-6 on UniProt.org