- Regulatory Status
- RUO
- Other Names
- P40, MEA (mast cell growth enhancing activity), MCGF (Mast cellgrowth factor), TCGF-3 (T cell growth factor-3)
-
Rat IL-9 induces proliferation of MC/9 mouse mast cells in a dose dose-dependent manner. The ED50 for this effect is 80 - 800 ng/mL. -
Stability Testing for Recombinant Rat IL-9. Recombinant rat IL-9 was aliquoted in PBS and pH 7.2. at 0.2 mg/mL. One aliquot was kept at 4°C (control), and another was frozen and thawed four times (4x Freeze/Thaw). The samples were tested by their ability to induce the proliferation of MC/9 mouse mast cells in a dose-dependent manner. The ED50 for this effect is 80 – 800 ng/mL.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 569804 | 25 µg | $590.00 | |||
| 569806 | 100 µg | $1110.00 | |||
Interleukin-9 (IL-9) regulates variety of hematopoietic cells, stimulates cell proliferation, and prevents apoptosis. Mature rat IL-9 shows 58% and 74% amino acid sequence homology with human and mouse IL-9, respectively. The IL-9 receptor consists of the cytokine-specific IL-9 receptor α-chain (IL-9Rα) and the common γ-chain that is shared by IL-2, IL-4, IL-7, IL-15 and IL-21. IL-9 is expressed by activated Th9, Th17, Treg, and mast cells and appears to play important roles in the development and function of subsets within the CD4+ T cell lineage. IL-9 also inhibits immune responses by enhancing the suppressive properties of Tregs and by recruiting immune suppressive mast cells to sites of inflammation. Genetic studies on a mouse model of asthma demonstrated that IL-9 plays a key role in the pathogenesis of bronchial hyperresponsiveness correlating with the observed increased expression of IL-9 and IL-9R in lungs of asthmatic patients. In addition, IL-9 promotes mast cell mediated intestinal permeability, development of food allergies and atopic dermatitis.
Product Details
- Source
- Recombinant rat IL-9, amino acid Gln19-Ala144 (Accession No. D4A819) was expressed in 293E.
- Molecular Mass
- The 126 amino acid recombinant protein has a predicted molecular mass of approximately 14.1 kD. The DTT-reduced and non-reduced protein migrate at approximately 27 - 35 kD by SDS-PAGE. The predicted N-terminal amino acid is Gln.
- Purity
- > 95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS, pH7.2
- Endotoxin Level
- Less than 0.1 EU per µg protein as determined by the LAL method.
- Concentration
- 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- ED50 = 80 – 800 ng/mL, as determined by a dose-dependent stimulation in a proliferation assay with MC/9 cells. Deep Blue Cell Viability™ Kit (Cat. No. 424701) is used to measure the proliferation.
- Application
-
Bioassay
- Application Notes
-
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Monomer
- Distribution
-
Th2 cells, mast cells, activated Th9, Th17, and Treg cells
- Function
- Autocrine growth and activation factor for Th17, Tregs, and mast cells. IL-1, histamine, and LPS induce the expression of IL-9 in mast cells. IL-9 is induced synergistically by IL-2, IL-4, and TGFβ and inhibited by IFNγ in naÏve CD4 T cells
- Interaction
- T helper cells, Th9, Th17, Tregs, human airway smooth muscle cells
- Ligand/Receptor
- IL-9R complex (IL-9Rα and γ chain)
- Bioactivity
- rat IL-9 induces proliferation of MC/9 cells
- Cell Type
- Hematopoietic stem and progenitors
- Biology Area
- Cell Biology, Immunology, Stem Cells
- Molecular Family
- Cytokine/Chemokine Receptors
- Antigen References
-
- Goswami R & Kaplan MH. 2011. J Immunol. 186:3283-8.
- Kaiko GE & Foster PS. 2011. Curr Opin Allergy Clin Immunol. 11:39-45.
- Townsend JM, et al. 2000. Immunity. 13:573-83.
- Mock BA, et al. 1990. Immunogenetics. 31:265-70.
- Tan C, et al. 2010. J Immunol. 185:6795-801.
- Jäger A, et al. 2009. J Immunol. 183:7169-77.
- Sismanopoulos N, et al. 2012. PLoS One. 7(3): e33271.
- Ye D, et al. 2020. Life Sci. 255:117844.
- Gene ID
- 116558 View all products for this Gene ID
- UniProt
- View information about IL-9 on UniProt.org