Spark Blue™ 550 anti-human CD19 (Flexi-Fluor™) Antibody

Pricing & Availability
Clone
SJ25C1 (See other available formats)
Regulatory Status
RUO
Other Names
CD19 Molecule, CD19 Antigen, Differentiation Antigen CD19, B-Lymphocytes Surface Antigen B4, T-cell Surface Antigen Leu-12, CVID3, B4, B-lymphocyte Antigen CD19
Isotype
Mouse IgG1, κ
Compare all formats See Spark Blue™ 550 spectral data
Cat # Size Price Quantity
285017 50 µg $394.00
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Description

CD19 is a 95 kD type I transmembrane glycoprotein also known as B4. It is a member of the immunoglobulin superfamily expressed on B cells (from pro-B to blastoid B cells, absent on plasma cells) and follicular dendritic cells. CD19 is involved in B cell development, activation, and differentiation. CD19 forms a complex with CD21 (CR2) and CD81 (TAPA-1), and functions as a BCR co-receptor.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
NALM1 + NALM16 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Spark Blue™ 550 under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC
Recommended Usage
Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.
Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone SJ25C1 partially blocks anti-human CD19 clones HIB19 and 4G7 staining based on in-house testing

Additional Product Notes

For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.

RRID
AB_3106282 (BioLegend Cat. No. 285017)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 95 kD.
Distribution

B lineage (except plasma cells), follicular dendritic cells.

Function
B cell activation and differentiation.
Interaction
Forms a complex with CD21, CD81 and CD225 in the membrane of mature B cells and interacts with VAV and LYN. It also interacts with GRAB2 and SOS when phosphorylated on Tyr-348 and/or Tyr-378 and with PLCG2 when phosphorylated on Tyr-409.
Ligand/Receptor
Forms complex with CD21 (CR2) and CD81 (TAPA-1), BCR co-receptor.
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Tedder T, et al. 1994. Immunol. Today 15:437.
2. Bradbury L, et al. 1993. J. Immunol. 151:2915.

Gene ID
930 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org
Go To Top Version: 1    Revision Date: 02/06/2024

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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