- Regulatory Status
- RUO
- Other Names
- Streptavidin-Spark Blue™, SAv-Spark Blue™ 550, streptavidin Spark Blue™ 550
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Human peripheral blood lymphocytes were stained with biotinylated anti-human CD3, followed by SAV-Spark Blue™ 550 and anti-human CD19 APC (left) or biotinylated mouse IgG1, κ isotype control, followed with SAV-Spark Blue™ 550 and anti-human CD19 APC (right).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 405363 | 25 µg | $106.00 | |||
| 405364 | 100 µg | $253.00 | |||
Streptavidin is a 52.8 kDa tetrameric protein obtained from Streptomyces avidinii. It binds to biotin with a very high affinity and is one of the strongest interactions in nature with a dissociation constant of 10-14 mol/L. It is used to detect biotinylated proteins in a wide range of applications.
Product Details
- Verified Reactivity
- Human, Mouse, Rat, All Species
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- Streptavidin is conjugated with Spark Blue™ 550 under optimal conditions
- Concentration
- 0.5 mg/mL (concentration relates to the Streptavidin only component of the conjugate)
- Storage & Handling
- The streptavidin-Spark Blue™ 550 solution should be stored undiluted between 2°C and 8°C, and protected from exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this Streptavidin-Spark Blue™ 550 is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the Spark Blue™ 550 conjugation. For immunofluorescent staining, the recommended use of this reagent is ≤ 1 µg per 106 cells in 100 µL staining volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Spark Blue™ 550 has a maximum excitation of 516 nm and a maximum emission of 540 nm. - Excitation Laser
-
Blue Laser (488 nm)
Antigen Details
- Gene ID
- NA
- UniProt
- View information about Biotin on UniProt.org