- Clone
- PC61 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- IL-2Rα, Ly-43, p55, Tac
- Isotype
- Rat IgG1, λ
| Cat # | Size | Price | Quantity | |||
|---|---|---|---|---|---|---|
| 285179 | 50 µg | $336.00 | ||||
CD25 is a 55 kD glycoprotein also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subsets, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132 (common γ chain), CD25 forms the high affinity signaling IL-2 receptor.
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- IL-2-dependent cytolytic mouse T-cell clone B6.1
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Spark Blue™ 574 under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC
- Recommended Usage
-
Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.
For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration. - Excitation Laser
-
Blue Laser (488 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, in vitro blocking of IL-2 binding to low- and high-affinity receptors1-4, growth inhibition of IL-2-dependent T-cell lines1-4, in vivo depletion of CD25+CD4+ Treg cells5-8,10, and immunohistochemical staining of acetone-fixed frozen sections2. PC61 antibody recognizes a different epitope than 3C7 antibody (Cat. No. 101902). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 102040) with endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered.
- Additional Product Notes
-
For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.
-
Application References
(PubMed link indicates BioLegend citation) -
- Lowenthal JW, et al. 1985. Nature 315:669. (IP, Block)
- Ceredig R, et al. 1985. Nature 314:98. (IP, IHC, Block)
- Lowenthal JW, et al. 1985. J. Immunol. 135:3988. (Block)
- Moreau JL, et al. 1987. Eur. J. Immunol. 17:929. (Block)
- Takahashi T, et al. 2000. J. Exp. Med. 192:303. (Deplete)
- Onizuka S, et al. 1999. Cancer Res. 59:3128. (Deplete)
- Lei TC, et al. 2005. Blood 105:4865. (Deplete)
- Pasare C, et al. 2004. Immunity 21:733. (Deplete)
- León-Ponte M, et al. 2007. Blood 109:3139.
- Cao OW, et al. 2007. Blood doi:10.1182/blood-2007-02-073304. (Deplete)
- Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719.
- Liu F, et al. 2011. Arch Toxicol. 85:1383. PubMed
- Anguela XM, et al. 2013. Diabetes. 62:551. PubMed
- RRID
-
AB_3662519 (BioLegend Cat. No. 285179)
Antigen Details
- Structure
- Forms high affinity IL-2R with IL-2Rβ (CD122) and IL-2Rγ (CD132), 55 kD
- Distribution
-
Activated T cells and B cells, thymocyte subset, pre-B cells, T regulatory cells
- Function
- IL-2 receptor
- Ligand/Receptor
- IL-2
- Cell Type
- B cells, T cells, Thymocytes, Tregs
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
- Taniguchi T, et al. 1993. Cell 73:5-8.
- Waldmann TA. 1991. J Biol Chem. 266:2681-4.
- Read S, et al. 2000. J Exp Med. 192:295-302.
- Lowenthal JW, et al. 1985. J Immunol. 135:3988-94.
- Gene ID
- 16184 View all products for this Gene ID
- UniProt
- View information about CD25 on UniProt.org
Other Formats
View All CD25 Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-mouse CD25
Con A-stimulated (3 days)splenocytes stained with PC61 APC -
Biotin anti-mouse CD25
Con A-stimulated C57BL/6 splenocytes (3 days) stained with ... -
FITC anti-mouse CD25
Con-A Stimulated (3 days) BALB/c mouse splenocytes stained w... -
PE anti-mouse CD25
Con A-stimulated (3 days) BALB/c mouse splenocytes stained w... -
PE/Cyanine5 anti-mouse CD25
Con A-stimulated (day-3) C57BL/6 mouse splenocytes stained w... -
Purified anti-mouse CD25
C57BL/6 mouse splenocytes stained with purified PC61, follow... 
Fresh, frozen mouse spleen was stained with purified CD25 cl... -
PE/Cyanine7 anti-mouse CD25
Con A-stimulated (2 days) BALB/c mouse splenocytes stained w... -
Alexa Fluor® 488 anti-mouse CD25
Con A-stimulated (3 days) BALB/c mouse splenocytes stained w... -
Alexa Fluor® 647 anti-mouse CD25
Con A-stimulated (3 days) BALB/c mouse splenocytes stained w... -
Pacific Blue™ anti-mouse CD25
Con A-stimulated (3 days) C57BL/6 mouse splenocytes stained ... -
Alexa Fluor® 700 anti-mouse CD25
Con A-stimulated (day-3) C57BL/6 mouse splenocytes stained w... -
APC/Cyanine7 anti-mouse CD25
Con A-stimulated (day-3) C57BL/6 mouse splenocytes stained w... -
PerCP/Cyanine5.5 anti-mouse CD25
Con A-stimulated C57BL/7 splenocytes (Day 3) stained with PC... -
PerCP anti-mouse CD25
Con A-stimulated C57BL/7 splenocytes (Day 3) stained with P... -
Brilliant Violet 421™ anti-mouse CD25
C57BL/6 mouse splenocytes were stained with CD4 FITC and CD2... 
-
Brilliant Violet 605™ anti-mouse CD25
C57BL/6 mouse splenocytes were stained with CD4 PE and CD25 ... -
Brilliant Violet 650™ anti-mouse CD25
C57BL/6 mouse splenocytes were stained with CD4 FITC and CD2... -
Ultra-LEAF™ Purified anti-mouse CD25
Con A-stimulated (3 days) BALB/c mouse splenocytes stained w... -
Brilliant Violet 510™ anti-mouse CD25
Con A-stimulated (3 days) Balb/c splenocytes were stained wi... -
PE/Dazzle™ 594 anti-mouse CD25
Con A-stimulated (3 days) C57BL/6 mouse splenocytes were sta... -
Brilliant Violet 711™ anti-mouse CD25
Con A-stimulated (3 days) C57BL/6 mouse splenocytes were sta... -
Brilliant Violet 785™ anti-mouse CD25
Con A-stimulated (3 days) C57BL/6 mouse splenocytes were sta... -
Alexa Fluor® 594 anti-mouse CD25
C57BL/6 mice were intraperitoneally injected with 100 µg LEA... -
APC/Fire™ 750 anti-mouse CD25
C57BL/6 mouse splenocytes were stained with CD4 PE and CD25 ... 
-
TotalSeq™-A0097 anti-mouse CD25
-
KIRAVIA Blue 520™ anti-mouse CD25
Con A-stimulated (3 days) C57BL/6 mouse splenocytes were sta... 
C57BL/6 mouse splenocytes were stained with CD4 APC and CD25... -
TotalSeq™-B0097 anti-mouse CD25
-
TotalSeq™-C0097 anti-mouse CD25
-
Spark NIR™ 685 anti-mouse CD25 Antibody
Con A-stimulated (day-3) C57BL/6 mouse splenocytes were stai... -
PE/Fire™ 640 anti-mouse CD25
Con A-stimulated (3 days) C57BL/6 mouse splenocytes were sta... 
C57BL/6 mouse splenocytes were stained with anti-mouse CD4 F... -
Spark YG™ 581 anti-mouse CD25
C57BL/6 mouse splenocytes were stained with anti-mouse CD4 P... -
APC/Fire™ 810 anti-mouse CD25
Con A-stimulated (3 days) C57BL/6 mouse splenocytes were sta... -
Brilliant Violet 750™ anti-mouse CD25
Mouse (C57BL/6) splenocytes were stained with anti-mouse CD4... -
PerCP/Fire™ 780 anti-mouse CD25
C57BL/6 mouse splenocytes were stained with anti-mouse CD4 A... -
PE/Fire™ 700 anti-mouse CD25
C57BL/6 mouse splenocytes were stained with anti-mouse CD4 (... -
Spark PLUS UV395™ anti-mouse CD25
C57BL/6 mouse splenocytes were stained with anti-mouse CD4 (... -
Spark Blue™ 574 anti-mouse CD25 (Flexi-Fluor™)