- Clone
- A18246G (See other available formats)
- Regulatory Status
- RUO
- Other Names
- IL-2Rα, Ly-43, p55, Tac
- Isotype
- Rat IgG2b, κ
-
C57BL/6 mouse splenocytes were stained with anti-mouse CD4 (clone GK1.5) FITC and anti-mouse CD25 (clone A18246G) Spark PLUS UV395™ (left) or rat IgG2b, κ Spark PLUS UV395™ isotype control (right).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 112605 | 25 µg | $170.00 | |||
| 112606 | 100 µg | $350.00 | |||
CD25, also known as the low affinity IL-2Rα, Ly-43, p55, or Tac, is a 55 kD glycoprotein. It is expressed on activated T and B cells, thymocyte subsets, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132 (common γ chain), CD25 forms the high affinity signaling IL-2 receptor. CD25 plays a role in lymphocyte differentiation and activation/proliferation.
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Spark PLUS UV395™ under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Spark PLUS UV395™ has a maximum excitation of 355 nm and a maximum emission of 385 nm. - Excitation Laser
-
Ultraviolet Laser (355 nm)
- RRID
-
AB_3662172 (BioLegend Cat. No. 112605)
AB_3662172 (BioLegend Cat. No. 112606)
Antigen Details
- Structure
- Forms high affinity IL-2R with IL-2Rβ (CD122) and IL-2Rγ (CD132), 55 kD
- Distribution
-
Activated T cells and B cells, thymocyte subset, pre-B cells, T regulatory cells
- Function
- IL-2 receptor
- Ligand/Receptor
- IL-2
- Cell Type
- B cells, T cells, Tregs
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
- Taniguchi T, et al. 1993. Cell. 73:5-8.
- Waldmann TA. 1991. J Biol Chem. 266:2681-4.
- Read S, et al. 2000. J Exp Med. 192:295-302.
- Lowenthal JW, et al. 1985. J Immunol. 135:3988-94.
- Gene ID
- 16184 View all products for this Gene ID
- UniProt
- View information about CD25 on UniProt.org
Other Formats
View All CD25 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Ultra-LEAF™ Purified anti-mouse CD25 | A18246G | FC,IP |
| Spark PLUS UV395™ anti-mouse CD25 | A18246G | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Ultra-LEAF™ Purified anti-mouse CD25
250 µg of Ultra-LEAF™ Purified anti-mouse CD25 antibody (clo... 
% CD3+CD4+CD25+ splenocytes analyzed in mice intraperitoneal... -
Spark PLUS UV395™ anti-mouse CD25
C57BL/6 mouse splenocytes were stained with anti-mouse CD4 (...