Spark Red™ 718 anti-human CD11b (Flexi-Fluor™) Antibody

Pricing & Availability
Clone
ICRF44 (See other available formats)
Regulatory Status
RUO
Workshop
IV M047
Other Names
Integrin αM chain, C3biR, CR3, Mac-1, Mo1, ITGAM
Isotype
Mouse IgG1, κ
Compare all formats See Spark Red™ 718 spectral data
Cat # Size Price Quantity
285077 50 µg $375.00
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Description

CD11b is a 165-170 kD type I transmembrane glycoprotein also known as αM integrin, Mac-1, CR3, and C3biR. CD11b non-covalently associates with integrin β2 (CD18) and is expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b/CD18 is critical for the transendothelial migration of monocytes and neutrophils. It is also involved in granulocyte adhesion, phagocytosis, and neutrophil activation. CD11b/CD18 interacts with ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen, and factor X.

Technical data sheet

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon, Chimpanzee, Common Marmoset, Pig
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Spark Red™ 718 under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC
Recommended Usage
Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.
Excitation Laser
Red Laser (633 nm)
Application Notes

The ICRF44 antibody inhibits heterotypic adhesion of granulocytes in response to fMLP. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections, immunofluorescence microscopy5, stimulation of monocytes3, blocking of heterotypic PMN aggregation8, and blocking of granulocyte activation12. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.

The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 301361 & 301362).

Additional Product Notes

For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
  2. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
  3. Rezzonico R, et al. 2001. Blood 97:2932. (Stim)
  4. Marsik C, et al. 2003. Shock 20:493. (FC)
  5. David A, et al. 2003. J. Leukoc. Biol. 74:551. (IF)
  6. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  7. Thurlow LR, et al. 2010. Infect. Immun. 128:1128. (FC) PubMed
  8. Jadhav S, et al. 2001. J. Immunol. 167:5986. (Block)
  9. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  10. Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21. (FC)
  11. Wen T, et al. 2014. J Immunol. 192:5481. (FC) PubMed
  12. Sprong T, et al. 2003. Blood 102:3702. (Block)
  13. Cash JL, et al. 2013. EMBO Rep. 14:999. (FC) PubMed
  14. Larsson K, et al. 2015. PNAS. PubMed
RRID
AB_3106334 (BioLegend Cat. No. 285077)

Antigen Details

Structure
Integrin, type I transmembrane glycoprotein, associates with integrin β2 (CD18), 165-170 kD
Distribution

Granulocytes, monocytes/macrophages, dendritic cells, NK cells, subset of T cells, subset of B cells

Function
Adhesion, phagocytosis, chemotaxis, neutrophil activation
Ligand/Receptor
ICAM-1(CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen, factor X
Cell Type
B cells, Dendritic cells, Granulocytes, Macrophages, Monocytes, Neutrophils, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Stewart M, et al. 1995. Curr Opin Cell Biol. 7:690.

Gene ID
3684 View all products for this Gene ID
UniProt
View information about CD11b on UniProt.org

Other Formats

View All CD11b Reagents Request Custom Conjugation
Description Clone Applications
APC anti-human CD11b ICRF44 FC
Biotin anti-human CD11b ICRF44 FC
PE anti-human CD11b ICRF44 FC
PE/Cyanine5 anti-human CD11b ICRF44 FC
Purified anti-human CD11b ICRF44 FC,CyTOF®,ICC,IHC
Pacific Blue™ anti-human CD11b ICRF44 FC
Alexa Fluor® 488 anti-human CD11b ICRF44 FC
Alexa Fluor® 647 anti-human CD11b ICRF44 FC
PE/Cyanine7 anti-human CD11b ICRF44 FC
PerCP/Cyanine5.5 anti-human CD11b ICRF44 FC
Brilliant Violet 421™ anti-human CD11b ICRF44 FC,ICC
Brilliant Violet 570™ anti-human CD11b ICRF44 FC
FITC anti-human CD11b ICRF44 FC
Brilliant Violet 605™ anti-human CD11b ICRF44 FC
Brilliant Violet 510™ anti-human CD11b ICRF44 FC,ICC
Brilliant Violet 650™ anti-human CD11b ICRF44 FC
Purified anti-human CD11b (Maxpar® Ready) ICRF44 FC,CyTOF®
Alexa Fluor® 594 anti-human CD11b ICRF44 ICC,FC
APC/Cyanine7 anti-human CD11b ICRF44 FC
Brilliant Violet 711™ anti-human CD11b ICRF44 FC
Brilliant Violet 785™ anti-human CD11b ICRF44 FC
PE/Dazzle™ 594 anti-human CD11b ICRF44 FC
APC/Fire™ 750 anti-human CD11b ICRF44 FC
APC anti-human CD11b ICRF44 FC
PE anti-human CD11b ICRF44 FC
TotalSeq™-A0161 anti-human CD11b ICRF44 PG
Alexa Fluor® 700 anti-human CD11b ICRF44 FC
PE/Cyanine7 anti-human CD11b ICRF44 FC
TotalSeq™-B0161 anti-human CD11b ICRF44 PG
TotalSeq™-C0161 anti-human CD11b ICRF44 PG
PerCP/Cyanine5.5 anti-human CD11b ICRF44 FC
Ultra-LEAF™ Purified anti-human CD11b ICRF44 FC,CyTOF®,Block,ICC,IHC
TotalSeq™-D0161 anti-human CD11b ICRF44 PG
GMP PE/Cyanine7 anti-human CD11b ICRF44 FC
Pacific Blue™ anti-human CD11b ICRF44 FC
GMP PE anti-human CD11b ICRF44 FC
Spark UV™ 387 anti-human CD11b ICRF44 FC
GMP PerCP/Cyanine5.5 anti-human CD11b ICRF44 FC
FITC anti-human CD11b ICRF44 FC
APC/Fire™ 750 anti-human CD11b ICRF44 FC
GMP APC anti-human CD11b ICRF44 FC
GMP FITC anti-human CD11b ICRF44 FC
GMP APC/Fire™ 750 anti-human CD11b ICRF44 FC
Spark Blue™ 515 anti-human CD11b ICRF44 FC
Spark Violet™ 500 anti-human CD11b ICRF44 FC
Spark Red™ 718 anti-human CD11b (Flexi-Fluor™) ICRF44 FC
Go To Top Version: 1    Revision Date: 02/07/2024

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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