Spark Red™ 718 anti-human CD159a (NKG2A) (Flexi-Fluor™) Antibody

Pricing & Availability
Clone
S19004C (See other available formats)
Regulatory Status
RUO
Other Names
Killer Cell Lectin Like Receptor C1, KLRC1, NK Cell Receptor A
Isotype
Mouse IgG1, κ
Compare all formats See Spark Red™ 718 spectral data
Cat # Size Price Quantity
285106 50 µg $375.00
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Description

CD159a, also known as NKG2A or KLRC1, is a type II transmembrane protein. It belongs to the killer cell lectin-like receptor family also known as the NKG2 family. It is expressed on NK and NKT cells and T cell subsets. NKG2A is an inhibitory NK cell receptor and it is expressed as a heterodimer with CD94. In human, NKG2A/CD94 complex interacts HLA-E on target cells and inhibits T cells and NK cell effector functions. Recent studies showed that NKG2A blockade enhances the anti-tumor effect of NK cell and CD8+ T cells.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human NKG2A/CD94-transfectants
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Spark Red™ 718 under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC
Recommended Usage
Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.
Excitation Laser
Red Laser (633 nm)
Application Notes

Clone S19004C does not cross-react with mouse CD159a.

Additional Product Notes

For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.

RRID
AB_3106358 (BioLegend Cat. No. 285106)

Antigen Details

Structure
Type II transmembrane proteins of the C-type lectin superfamily
Distribution

NK cells, NKT cells, T cell subsets

Function
Inhibiting NK cell receptor
Ligand/Receptor
HLA-E
Cell Type
Lymphocytes, NK cells, NKT cells, T cells
Biology Area
Immuno-Oncology, Immunology
Molecular Family
Immune Checkpoint Receptors
Antigen References
  1. Brooks AG, et al. 1999. J Immunol. 162:305-13.
  2. Kaiser BK, et al. 2008. Proc Natl Acad Sci U S A. 105: 6696-701.
  3. Braud VM, et al. 2003. Trends Immunol. 24:162-4.
  4. Kamiya T, et al. 2019. J Clin Invest. 129:2094.
  5. André P, et al. 2018. Cell. 175:1731.
Gene ID
3821 View all products for this Gene ID
UniProt
View information about CD159a on UniProt.org

Other Formats

View All CD159a Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-human CD159a (NKG2A) S19004C FC
PE anti-human CD159a (NKG2A) S19004C FC
APC anti-human CD159a (NKG2A) Antibody S19004C FC
Pacific Blue™ anti-human CD159a (NKG2A) Antibody S19004C FC
PE/Cyanine7 anti-human CD159a (NKG2A) Antibody S19004C FC
Alexa Fluor® 647 anti-human CD159a (NKG2A) Antibody S19004C FC
PE/Cyanine5 anti-human CD159a (NKG2A) S19004C FC
APC/Fire™ 750 anti-human CD159a (NKG2A) S19004C FC
Alexa Fluor® 700 anti-human CD159a (NKG2A) Antibody S19004C FC
PE/Dazzle™ 594 anti-human CD159a (NKG2A) Antibody S19004C FC
Biotin anti-human CD159a (NKG2A) Antibody S19004C FC
Alexa Fluor® 488 anti-human CD159a (NKG2A) S19004C FC
PerCP/Cyanine5.5 anti-human CD159a (NKG2A) S19004C FC
FITC anti-human CD159a (NKG2A) S19004C FC
TotalSeq™-C1374 anti-human CD159a (NKG2A) S19004C PG
TotalSeq™-D1374 anti-human CD159a (NKG2A) S19004C PG
TotalSeq™-A1374 anti-human CD159a (NKG2A) S19004C PG
APC/Fire™ 810 anti-human CD159a (NKG2A) S19004C FC
Brilliant Violet 421™ anti-human CD159a (NKG2A) S19004C FC
Brilliant Violet 605™ anti-human CD159a (NKG2A) S19004C FC
Brilliant Violet 785™ anti-human CD159a (NKG2A) S19004C FC
TotalSeq™-B1374 anti-human CD159a (NKG2A) S19004C PG
PerCP/Fire™ 806 anti-human CD159a (NKG2A) S19004C FC
PerCP/Fire™ 780 anti-human CD159a (NKG2A) S19004C FC
PE/Fire™ 810 anti-human CD159a (NKG2A) S19004C FC
APC/Cyanine7 anti-human CD159a (NKG2A) S19004C FC
Brilliant Violet 650™ anti-human CD159a (NKG2A) S19004C FC
Spark Red™ 718 anti-human CD159a (NKG2A) (Flexi-Fluor™) S19004C FC
Brilliant Violet 711™ anti-human CD159a (NKG2A) S19004C FC
Go To Top Version: 1    Revision Date: 02/07/2024

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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